Biochemical Composition and Immunological Comparison of Select Pecan [Carya illinoinensis (Wangenh.) K. Koch] Cultivars
| Autor: | Tommy E. Thompson, David Heber, Navindra P. Seeram, Harshal H. Kshirsagar, Mahesh Venkatachalam, Shridhar K. Sathe, Kenneth H. Roux |
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| Rok vydání: | 2007 |
| Předmět: |
Nitrogen
Flavonoid Carbohydrates Antibodies food Species Specificity Botany Nuts Tannin Food science Cultivar Sugar Chemical composition Carya Plant Proteins chemistry.chemical_classification biology Juglandaceae Carya illinoinensis General Chemistry biology.organism_classification food.food chemistry Polyphenol Fatty Acids Unsaturated Electrophoresis Polyacrylamide Gel General Agricultural and Biological Sciences Tannins Food Analysis |
| Zdroj: | Journal of Agricultural and Food Chemistry. 55:9899-9907 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf0714721 |
| Popis: | On an edible portion basis, pecan moisture, protein, lipid, total soluble sugars, and ash contents ranged from 2.1% to 6.4%, 6.0% to 11.3%, 65.9% to 78.0%, 3.3% to 5.3%, and 1.2% to 1.8%, respectively. With the exception of a high tannin (2.7%) Texas seedling, pecan tannin content was in a narrow range (0.6-1.85%). Unsaturated fatty acids (>90%) dominated pecan lipid composition with oleic (52.52-74.09%) and linoleic (17.69-37.52%) acids as the predominant unsaturated fatty acids. Location significantly influenced pecan biochemical composition. Pecan lipid content was negatively correlated with protein (r = -0.663) and total sugar (r = -0.625). Among the samples tested using SDS-PAGE a common pattern, with minor differences, in subunit polypeptide profiles was revealed. Rabbit polyclonal antibody-based immunoblotting experiments (Western blot) also illustrated the similarity in polypeptide profiles with respect to immunoreactivity. All tested cultivars registered similar immunoreactivity when their protein extracts (each at 1 mg/mL) were assessed using inhibition ELISAs (mean +/- standard deviation = 0.89 +/- 0.20; n = 27) with the USDA "Desirable" cultivar as the reference standard (immunoreactivity designated as 1.0). |
| Databáze: | OpenAIRE |
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