Tailoring Interleaflet Lipid Transfer with a DNA-based Synthetic Enzyme
| Autor: | Sobota, Diana, Joshi, Himanshu, Ohmann, Alexander, Aksimentiev, Aleksei, Keyser, Ulrich F |
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| Přispěvatelé: | Sobota, Diana [0000-0001-5909-2876], Joshi, Himanshu [0000-0003-0769-524X], Ohmann, Alexander [0000-0003-3537-1074], Aksimentiev, Aleksei [0000-0002-6042-8442], Keyser, Ulrich F [0000-0003-3188-5414], Apollo - University of Cambridge Repository |
| Rok vydání: | 2020 |
| Předmět: |
Letter
Lipid Bilayers Bioengineering 02 engineering and technology lipid flipping DNA nanotechnology General Materials Science A-DNA synthetic ion channel Membranes biology Artificial enzyme Chemistry Mechanical Engineering General Chemistry DNA 021001 nanoscience & nanotechnology Condensed Matter Physics molecular dynamics Membrane dodecane biology.protein Biophysics lipids (amino acids peptides and proteins) lipid membranes 0210 nano-technology Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Nano Letters |
| ISSN: | 1530-6984 |
| DOI: | 10.1021/acs.nanolett.0c00990 |
| Popis: | Lipid membranes, enveloping all living systems, are of crucial importance, and control over their structure and composition is a highly desirable functionality of artificial structures. However, the rational design of protein-inspired systems is still challenging. Here we have developed a highly functional nucleic acid construct that self-assembles and inserts into membranes, enabling lipid transfer between inner and outer leaflets. By designing the structure to account for interactions between the DNA, its hydrophobic modifications, and the lipids, we successfully exerted control over the rate of interleaflet lipid transfer induced by our DNA-based enzyme. Furthermore, we can regulate the level of lipid transfer by altering the concentration of divalent ions, similar to stimuli-responsive lipid-flipping proteins. |
| Databáze: | OpenAIRE |
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