Effects of acute and repeated restraint stress on corticotropin-releasing hormone binding protein mRNA in rat amygdala and dorsal hippocampus
| Autor: | David Hsu, Jay S. Balachandran, K. A. Lombardo, Vaishali P. Bakshi, Ned H. Kalin, Ryan J. Herringa, Patrick H. Roseboom |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Restraint Physical endocrine system medicine.medical_specialty Corticotropin-Releasing Hormone Central nervous system Hippocampus In situ hybridization Biology Amygdala Rats Sprague-Dawley Corticotropin-releasing hormone Limbic system Adrenocorticotropic Hormone Stress Physiological Internal medicine polycyclic compounds medicine Animals Homeostasis RNA Messenger Receptor Neurons General Neuroscience Rats medicine.anatomical_structure Endocrinology nervous system Carrier Proteins hormones hormone substitutes and hormone antagonists Basolateral amygdala |
| Zdroj: | Neuroscience Letters. 302:81-84 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/s0304-3940(01)01680-9 |
| Popis: | Corticotropin-releasing hormone (CRH) mediates endocrine, behavioral, and autonomic responses to stress. In addition to binding to two receptor subtypes, CRH binds to a CRH-binding protein (CRH-BP). While CRH-BP is hypothesized to play a role in regulating levels of free CRH and modulating the stress response, the effects of stressors on brain CRH-BP are relatively unexplored. The present study determined effects of acute and repeated restraint on CRH-BP mRNA in basolateral amygdala (BLA) and dorsal hippocampus (DH), brain regions involved in fear and motivation. Using in situ hybridization, we found that a single acute period of restraint significantly increased CRH-BP mRNA in BLA by 20% but had no effect in DH. Repeated restraint had no effect on basal levels of CRH-BP mRNA in BLA or DH. Importantly, repeated restraint blocked the effects of acute restraint in the BLA. These results demonstrate differential effects of acute and repeated restraint on CRH-BP mRNA. |
| Databáze: | OpenAIRE |
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