Rapid screening of RNA silencing suppressors by using a recombinant virus derived from beet necrotic yellow vein virus
| Autor: | Véronique Ziegler-Graff, D. Bortolamiol, Salah Bouzoubaa, G. Jonard, H. Guilley |
|---|---|
| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA) |
| Rok vydání: | 2009 |
| Předmět: |
Gene Expression Regulation
Viral 0106 biological sciences viruses [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Recombinant virus 01 natural sciences Benyvirus Virus Plant Viruses 03 medical and health sciences Virology Plant virus Tobacco mosaic virus Beet necrotic yellow vein virus Chenopodium quinoa Movement protein ComputingMilieux_MISCELLANEOUS Plant Diseases 030304 developmental biology 0303 health sciences food and beverages biology.organism_classification 3. Good health Plant Leaves RNA silencing RNA Viral RNA Interference Reassortant Viruses 010606 plant biology & botany |
| Zdroj: | Journal of General Virology Journal of General Virology, Microbiology Society, 2009, 90, pp.2536-2541 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.011213-0 |
| Popis: | To counteract plant defence mechanisms, plant viruses have evolved to encode RNA silencing suppressor (RSS) proteins. These proteins can be identified by a range of silencing suppressor assays. Here, we describe a simple method using beet necrotic yellow vein virus (BNYVV) that allows a rapid screening of RSS activity. The viral inoculum consisted of BNYVV RNA1, which encodes proteins involved in viral replication, and two BNYVV-derived replicons: rep3–P30, which expresses the movement protein P30 of tobacco mosaic virus, and rep5–X, which allows the expression of a putative RSS (X). This approach has been validated through the use of several known RSSs. Two potential candidates have been tested and we show that, in our system, the P13 protein of burdock mottle virus displays RSS activity while the P0 protein of cereal yellow dwarf virus-RPV does not. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|