DNA detection on transistor arrays following mutation-specific enzymatic amplification
| Autor: | Denis Côte, Ulrich Bockelmann, François Pouthas, Cédric Gentil |
|---|---|
| Přispěvatelé: | Laboratoire Pierre Aigrain (LPA), Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7)-Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Thomen, Philippe |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Materials science
Physics and Astronomy (miscellaneous) Silicon DNA field-effect transistor [SDV]Life Sciences [q-bio] chemistry.chemical_element 02 engineering and technology 010402 general chemistry 01 natural sciences law.invention chemistry.chemical_compound law medicine Nonsyndromic deafness Polymerase chain reaction business.industry Transistor Transistor array equipment and supplies 021001 nanoscience & nanotechnology medicine.disease Molecular biology 0104 chemical sciences [SDV] Life Sciences [q-bio] chemistry Mutation (genetic algorithm) Optoelectronics 0210 nano-technology business DNA |
| Zdroj: | Applied Physics Letters Applied Physics Letters, 2004, 84, pp.1594 Applied Physics Letters, American Institute of Physics, 2004, 84, pp.1594 |
| ISSN: | 0003-6951 |
| Popis: | An integrated array of silicon field-effect transistor structures is used for electronic detection of label-free DNA. Measurements of the dc current–voltage characteristics of the transistors gives us access to reproducible detection of single- and double-stranded DNA, locally adsorbed on the surface of the device. We combine this approach with allele-specific polymerase chain reaction, to test for the 35delG mutation, a frequent mutation related to prelingual nonsyndromic deafness. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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