Slc15a4, AP-3, and Hermansky-Pudlak syndrome proteins are required for Toll-like receptor signaling in plasmacytoid dendritic cells
| Autor: | Xiaohong Li, Amanda L. Blasius, Pei Lin, Yu Xia, Nora G. Smart, Carrie N. Arnold, Bruce Beutler, Charles Ross, Philippe Georgel, Sophie Rutschmann |
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| Rok vydání: | 2010 |
| Předmět: |
Adaptor Protein Complex 3
Vesicular Transport Proteins Nerve Tissue Proteins Biology Proinflammatory cytokine Mice Interferon Lectins medicine Animals Genetic Testing Receptor Toll-like receptor Multidisciplinary Cell Membrane Toll-Like Receptors Intracellular Signaling Peptides and Proteins Chromosome Mapping Membrane Transport Proteins TLR9 hemic and immune systems Dendritic Cells TLR7 Biological Sciences Cell biology Protein Transport Hermanski-Pudlak Syndrome Interferon Type I Mutation Signal transduction Carrier Proteins Interferon type I Signal Transduction medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 107:19973-19978 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1014051107 |
| Popis: | Despite their low frequency, plasmacytoid dendritic cells (pDCs) produce most of the type I IFN that is detectable in the blood following viral infection. The endosomal Toll-like receptors (TLRs) TLR7 and TLR9 are required for pDCs, as well as other cell types, to sense viral nucleic acids, but the mechanism by which signaling through these shared receptors results in the prodigious production of type I IFN by pDCs is not understood. We designed a genetic screen to identify proteins required for the development and specialized function of pDCs. One phenovariant, which we named feeble , showed abrogation of both TLR-induced type I IFN and proinflammatory cytokine production by pDCs, while leaving TLR responses intact in other cells. The feeble phenotype was mapped to a mutation in Slc15a4 , which encodes the peptide/histidine transporter 1 (PHT1) and has not previously been implicated in pDC function. The identification of the feeble mutation led to our subsequent observations that AP-3, as well as the BLOC-1 and BLOC-2 Hermansky-Pudlak syndrome proteins are essential for pDC signaling through TLR7 and TLR9. These proteins are not necessary for TLR7 or TLR9 signaling in conventional DCs and thus comprise a membrane trafficking pathway uniquely required for endosomal TLR signaling in pDCs. |
| Databáze: | OpenAIRE |
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