Serum anti-AP3D1 antibodies are risk factors for acute ischemic stroke related with atherosclerosis
| Autor: | Masaaki Ito, Yoichi Yoshida, Norie Sawada, Shoichiro Tsugane, Yoshio Kobayashi, Yasuo Iwadate, Minoru Takemoto, Tomoo Matsutani, Shu Yang Li, Natsuko Shinmen, Hiromi Ashino, Kazumasa Yamagishi, Takaki Hiwasa, Go Tomiyoshi, Makoto Sumazaki, Hisahiro Matsubara, Hideyuki Kuroda, Masaaki Kubota, Ken-ichiro Goto, Toshio Machida, Hao Wang, Satoshi Yajima, Hirotaka Takizawa, Seiichiro Mine, Hiroyasu Iso, Yoshiro Maezawa, Hideaki Shimada, Mizuki Sata, Katsuro Iwase, Koutaro Yokote, Rika Nakamura, Eiichi Kobayashi, Sohei Kobayashi, Kazuyuki Matsushita, Fumio Nomura |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Colorectal cancer Adaptor Protein Complex 3 Science Disease Gastroenterology Article Serology 03 medical and health sciences 0302 clinical medicine Antigen Risk Factors Diabetes mellitus Internal medicine Medicine Humans Aged Autoantibodies Ischemic Stroke Aged 80 and over Multidisciplinary biology business.industry Area under the curve Diagnostic markers Middle Aged medicine.disease Atherosclerosis Adaptor Protein Complex delta Subunits Stroke 030104 developmental biology 030220 oncology & carcinogenesis Immunoglobulin G biology.protein Female Antibody business Kidney disease |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Atherosclerosis has been considered as the main cause of morbidity, mortality, and disability worldwide. The first screening for antigen markers was conducted using the serological identification of antigens by recombinant cDNA expression cloning, which has identified adaptor-related protein complex 3 subunit delta 1 (AP3D1) as an antigen recognized by serum IgG antibodies of patients with atherosclerosis. Serum antibody levels were examined using the amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) using a recombinant protein as an antigen. It was determined that the serum antibody levels against AP3D1 were higher in patients with acute ischemic stroke, transient ischemic attack , diabetes mellitus (DM), cardiovascular disease , chronic kidney disease (CKD), esophageal squamous cell carcinoma (ESCC), and colorectal carcinoma than those in the healthy donors. The area under the curve values of DM, nephrosclerosis type of CKD, and ESCC calculated using receiver operating characteristic curve analysis were higher than that of other diseases. Correlation analysis showed that the anti-AP3D1 antibody levels were highly associated with maximum intima-media thickness, which indicates that this marker reflected the development of atherosclerosis. The results of the Japan Public Health Center-based Prospective Study indicated that this antibody marker is deemed useful as risk factors for AIS. |
| Databáze: | OpenAIRE |
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