An Isolation Procedure for the Native alpha Chain of Bovine Hemoglobin. A Study of the Functional Properties of This Chain and Its Hybrid with the Human beta Chain

Autor:	Harry S. Rollema, S H De Bruin, J J Joordens
Rok vydání:	1977
Předmět:	Macromolecular Substances Chemistry Partial Pressure Kinetics Alpha (ethology) Plasma protein binding Hydrogen-Ion Concentration Biochemistry Oxygen Hemoglobins Affinity chromatography Chain (algebraic topology) Animals Humans Cattle Hemoglobin Beta (finance) Alpha chain Protein Binding
Zdroj:	European Journal of Biochemistry. 75:211-215
ISSN:	1432-1033 0014-2956
DOI:	10.1111/j.1432-1033.1977.tb11519.x
Popis:	In this paper we present a procedure for the isolation of the native bovine alpha chain. The method is based on affinity chromatography. The results show that the ligand-binding properties of the bovine alpha chain are almost identical to those of the human alpha chain. The hybrid alphaB2 betaH2 prepared by mixing bovine alpha chains and human beta chains shows ligand binding properties similar to those of human hemoglobin and different from those of bovine hemoglobin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5b4a423d3e35a3767fc76deca2131fc https://doi.org/10.1111/j.1432-1033.1977.tb11519.x Zobrazit plný text záznamu Plný text ve formátu PDF