Functional analysis of alcS, a gene of the alc cluster in Aspergillus nidulans
| Autor: | Michel Flipphi, Xavier Leschelle, Xavier Robellet, Christian Vélot, Béatrice Felenbok, Emmanuel Dequier |
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| Rok vydání: | 2006 |
| Předmět: |
Transcription
Genetic Amino Acid Motifs Genes Fungal Molecular Sequence Data Protein domain Gene Dosage Microbiology Aspergillus nidulans GPR1 Fungal Proteins Open Reading Frames Gene Expression Regulation Fungal Genetics Amino Acid Sequence RNA Messenger DNA Fungal Gene Conserved Sequence Alcohol dehydrogenase Regulator gene Base Sequence Sequence Homology Amino Acid biology Cell Membrane Structural gene Alcohol Dehydrogenase Membrane Proteins RNA Fungal Sequence Analysis DNA Aldehyde Dehydrogenase Blotting Northern biology.organism_classification Introns Mutagenesis Insertional Biochemistry Membrane protein Multigene Family biology.protein Gene Deletion |
| Zdroj: | Fungal Genetics and Biology. 43:247-260 |
| ISSN: | 1087-1845 |
| Popis: | The ethanol utilization pathway (alc system) of Aspergillus nidulans requires two structural genes, alcA and aldA, which encode the two enzymes (alcohol dehydrogenase and aldehyde dehydrogenase, respectively) allowing conversion of ethanol into acetate via acetyldehyde, and a regulatory gene, alcR, encoding the pathway-specific autoregulated transcriptional activator. The alcR and alcA genes are clustered with three other genes that are also positively regulated by alcR, although they are dispensable for growth on ethanol. In this study, we characterized alcS, the most abundantly transcribed of these three genes. alcS is strictly co-regulated with alcA, and encodes a 262-amino acid protein. Sequence comparison with protein databases detected a putative conserved domain that is characteristic of the novel GPR1/FUN34/YaaH membrane protein family. It was shown that the AlcS protein is located in the plasma membrane. Deletion or overexpression of alcS did not result in any obvious phenotype. In particular, AlcS does not appear to be essential for the transport of ethanol, acetaldehyde or acetate. Basic Local Alignment Search Tool analysis against the A. nidulans genome led to the identification of two novel ethanol- and ethylacetate-induced genes encoding other members of the GPR1/FUN34/YaaH family, AN5226 and AN8390. |
| Databáze: | OpenAIRE |
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