Cytoprotective effects of paeoniflorin are associated with translocator protein 18 kDa
| Autor: | Dan Tang, Yun-Yun Pan, Xu Liu, Qinghong Fan, Mei-Yan Huang, Zhi-Kun Qiu, Zhe Zhang, Ying-Yu Chen, Tao Zhu, Ji-Sheng Chen, Xiao-Fang Cheng, Yu-Lu Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Neuroactive steroid Trilostane Pharmacology Protective Agents Neuroprotection Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Glucosides Biosynthesis Translocator protein medicine Animals biology Chemistry Allopregnanolone General Medicine Isoquinolines Receptors GABA-A Paeoniflorin Cytoprotection Rats 030104 developmental biology Astrocytes Monoterpenes biology.protein Steroids Carrier Proteins 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Biomedicine & Pharmacotherapy. 107:19-23 |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2018.07.112 |
| Popis: | Paeoniflorin (PF) is one of the important active components in peony that are known to produce the neuroprotective effects. However, the involved cytoprotective factors on brain astrocytes are remain unclear. Translocator protein 18 kDa (TSPO) and its downstream neurosteroids biosynthesis play a significant role in cytoprotection. Based on these, the role of TSPO and neurosteroids biosynthesis in the cytoprotective effects of PF is evaluated. The astrocyte cells were cultured and AC-5216 (TSPO ligand) was selected as the positive control drug. The cytoprotective effects of PF and the levels of neurosteroids were quantified by water-soluble tetrazolium assay and enzyme linked immunosorbent assay, respectively. The cytoprotective activities of PF were relevant to neurosteroids (e.g. progsterone and allopregnanolone) biosynthesis, while these effects were totally blocked by PK11195, trilostane and finasteride, respectively. In summary, the cytoprotective effects of PF maybe mediated by TSPO and neurosteroids biosynthesis. The findings may provide the new insights into the cytoprotective effects of PF. |
| Databáze: | OpenAIRE |
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