Development of an indirect ELISA based on a recombinant chimeric protein for the detection of antibodies against bovine Babesiosis
| Autor: | Marisa Diana Farber, José Manuel Jaramillo Ortiz, Néstor Fabián Sarmiento, Sofía Ana María de la Fourniere, Silvina Elizabeth Wilkowsky, Valeria Noely Montenegro |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Anticuerpos 030231 tropical medicine Enfermedades de los Animales Tick BOVINE BABESIOSIS bovine babesiosis multi-epitope recombinant antigen indirect ELISA seroprevalence Antibodies Epitope Article Animal Diseases 03 medical and health sciences 0302 clinical medicine Antigen Babesiosis Seroprevalence lcsh:Veterinary medicine General Veterinary biology Ciencias Veterinarias SEROPREVALENCE INDIRECT ELISA Babesia bovis 030108 mycology & parasitology biology.organism_classification Virology MULTI-EPITOPE RECOMBINANT ANTIGEN CIENCIAS AGRÍCOLAS Ganado Bovino Babesia biology.protein Enzootic lcsh:SF600-1100 ELISA Cattle Antibody purl.org/becyt/ford/4.3 [https] purl.org/becyt/ford/4 [https] ELISA Indirecto |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Veterinary Sciences Veterinary Sciences, Vol 5, Iss 1, p 13 (2018) Veterinary Sciences; Volume 5; Issue 1; Pages: 13 Veterinary Sciences 5 (1) : 13 (2018) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| Popis: | The current method for Babesia spp. serodiagnosis based on a crude merozoite antigen is a complex and time-consuming procedure. An indirect enzyme-linked immunosorbent assay (iELISA) based on a recombinant multi-antigen of Babesia bovis (rMABbO) was developed for detection of antibodies in bovines suspected of infection with this parasite. The multi-antigen comprises gene fragments of three previously characterized B. bovis antigens: MSA-2c, RAP-1 and the Heat Shock protein 20 that are well-conserved among geographically distant strains. The cutoff value for the new rMABbo-iELISA was determined using 75 known-positive and 300 known-negative bovine sera previously tested for antibodies to B. bovis by the gold-standard ELISA which uses a merozoite lysate. A cutoff value of ≥35% was determined in these samples by receiver operator characteristic (ROC) curve analysis, showing a sensitivity of 95.9% and a specificity of 94.3%. The rMABbo-iELISA was further tested in a blind trial using an additional set of 263 field bovine sera from enzootic and tick-free regions of Argentina. Results showed a good agreement with the gold standard test with a Cohen's kappa value of 0.76. Finally, the prevalence of bovine babesiosis in different tick enzootic regions of Argentina was analyzed where seropositivity values among 68-80% were obtained. A certain level of cross reaction was observed when samples from B. bigemina infected cattle were analyzed with the new test, which can be attributed to shared epitopes between 2 of the 3 antigens. This new rMABbo-iELISA could be considered a simpler alternative to detect anti Babesia spp. antibodies and appears to be well suited to perform epidemiological surveys at the herd level in regions where ticks are present. Fil: Jaramillo Ortiz, José Manuel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Montenegro, Valeria Noely. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina Fil: de la Fourniere, Sofía Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina Fil: Sarmiento, Nestor Fabian. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Corrientes. Estación Experimental Agropecuaria Mercedes; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina |
| Databáze: | OpenAIRE |
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