Heteroplasmia de la mutación del ADN mitocondrial m.3243A>G en la diabetes y sordera de herencia materna
| Autor: | Pablo Olmos, Roger Gejman, Ricardo Fadic, José Luis Santos, Luis Rodrigo Cataldo, Susan V. Smalley, Alejandra Parada, Alberto Diez |
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| Přispěvatelé: | Reyes, Humberto |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Revista médica de Chile v.141 n.3 2013 SciELO Chile CONICYT Chile instacron:CONICYT |
| ISSN: | 0034-9887 |
| Popis: | Maternally Inherited Diabetes and Deafness (MIDD) is caused by mutations in mitochondrial DNA (mtDNA), mainly m.3243A>G. Severity, onset and clinical phenotype of MIDD patients are partially determined by the proportion of mutant mitochondrial DNA copies in each cell and tissue (heteroplasmy). The identification of MIDD allows a corred treatment with insulin avoiding drugs that may interfere with mitochondrial electron chain transpon. We estimated the degree of heteroplasmy of the mutation m.3243A>G from blood, saliva, hair root and a muscle biopsy using quantitative PCR (qPCR) in a femole adult patient. For this purpose, PCR producis were inserted in a vector creating plasmids with 3243A or G. Mutant and wild-type vectors were mixed in different proportions to create a calibration curve used to interpolate heteroplasmy percentages with qPCR threshold cycles. The proportions of m.3243A>G heteroplasmy were 62% (muscle), 14% (saliva), 6% (blood leukocytes) and 3% in hair root. Quantitative analysis of heteroplasmy showed marked variations in different tissues (highest in muscle and lowest in blood). Given the relatively high heteroplasmy found in saliva, this type of biological sample may represent an adequate non-invasive way for assessing the presence of m.3243A>G mutations in epidemiologic studies. |
| Databáze: | OpenAIRE |
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