Glucocorticoid sensitivity in Behcet's disease
| Autor: | J A M van Laar, R.A.M. Quax, Marwen Ghabra, J W Koper, R van Heerebeek, S W J Lamberts, Jasper H Kappen, J M W Hazes, R A Feelders, K Greiner, G R Wallace, Farida Fortune, M Stanford, P. M. van Hagen, M Soylu, Eldad Ben-Chetrit |
|---|---|
| Přispěvatelé: | Çukurova Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
business.industry Research Endocrinology Diabetes and Metabolism Behçet's syndrome Behcet's syndrome Single-nucleotide polymorphism Peripheral blood mononuclear cell Pathophysiology Endocrinology Glucocorticoid Sensitivity single nucleotide polymorphism Internal medicine Immunology glucocorticoid sensitivity Internal Medicine medicine Allele business Receptor Gene Glucocorticoid medicine.drug |
| Zdroj: | Endocrine Connections |
| Popis: | ObjectiveGlucocorticoid (GC) sensitivity is highly variable among individuals and has been associated with susceptibility to develop (auto-)inflammatory disorders. The purpose of the study was to assess GC sensitivity in Behçet's disease (BD) by studying the distribution of four GC receptor (GR) gene polymorphisms and by measuring in vitro cellular GC sensitivity.MethodsHealthy controls and patients with BD in three independent cohorts were genotyped for four functional GR gene polymorphisms. To gain insight into functional differences in in vitro GC sensitivity, 19 patients with BD were studied using two bioassays and a whole-cell dexamethasone-binding assay. Finally, mRNA expression levels of GR splice variants (GR-α and GR-β) were measured.ResultsHealthy controls and BD patients in the three separate cohorts had similar distributions of the four GR polymorphisms. The Bcll and 9β minor alleles frequency differed significantly between Caucasians and Mideast and Turkish individuals.At the functional level, a decreased in vitro cellular GC sensitivity was observed. GR number in peripheral blood mononuclear cells was higher in BD compared with controls. The ratio of GR-α/GR-β mRNA expression levels was significantly lower in BD.ConclusionsPolymorphisms in the GR gene are not associated with susceptibility to BD. However, in vitro cellular GC sensitivity is decreased in BD, possibly mediated by a relative higher expression of the dominant negative GR-β splice variant. This decreased in vitro GC sensitivity might play an as yet unidentified role in the pathophysiology of BD. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|