RANTES, IFN-γ, CCR1, and CCR5 mRNA Expression in Peripheral Blood, Lymph Node, and Bronchoalveolar Lavage Mononuclear Cells during Primary Simian Immunodeficiency Virus Infection of Macaques
| Autor: | O. Neildez, P Caufour, F Matheux, R Le Grand, Frédéric Théodoro, Dominique Dormont, Arnaud Chéret, Bruno Vaslin |
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| Rok vydání: | 1999 |
| Předmět: |
Chemokine
Receptors CCR5 T cell animal diseases viruses Receptors CCR1 Simian Acquired Immunodeficiency Syndrome Biology medicine.disease_cause Bronchoalveolar Lavage Peripheral blood mononuclear cell Interferon-gamma Immune system T-Lymphocyte Subsets Virology medicine Animals RNA Messenger Primary isolate Chemokine CCL5 virus diseases Simian immunodeficiency virus biology.organism_classification Kinetics Macaca fascicularis medicine.anatomical_structure Gene Expression Regulation Viral replication Immunology Lentivirus Leukocytes Mononuclear biology.protein Female Receptors Chemokine Simian Immunodeficiency Virus Lymph Nodes Follow-Up Studies |
| Zdroj: | Virology. 255(2):285-293 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1998.9558 |
| Popis: | Primary infection of macaques with pathogenic isolates of simian immunodeficiency virus (SIV) (as a model of HIV infection in humans) represents a unique opportunity to study early lentivirus/host interactions. We sought to determine whether there is a temporal relationship linking SIV replication and dissemination and the expression of the chemokine RANTES (regulated upon activation normal T cell expressed and secreted) and the SIV/HIV coreceptor CCR5 in different tissues during acute SIV infection of macaques. Four cynomolgus macaques were inoculated intravenously with a pathogenic primary isolate of SIVmac251. RT-PCR was used to monitor the expression of RANTES and CCR5 mRNA in fresh isolated mononuclear cells from blood, lymph node, and bronchoalveolar lavages. These expressions were compared to those of IFN-γ as an indicator of the development of the immune response and to another receptor for RANTES, CCR1, which is not described as a coreceptor for SIV/HIV-1 entry. An enhancement of CCR1/CCR5 mRNA expression was noticed during primary SIVmac251 infection of macaques, mainly in tissue. In the three different compartments investigated, IFN-γ and RANTES overexpression was noticed by the time of systemic viral replication containment. Our results put CCR5 and RANTES mRNA expression back in the context of inflammatory and immune responses to SIV primary infection. |
| Databáze: | OpenAIRE |
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