Impacts of fast production of afucosylated antibodies and Fc mutants in ExpiCHO-S™ for enhancing FcγRIIIa binding and NK cell activation

Autor: Xiaotian Zhong, Jennifer Schenk, Paul Sakorafas, John Chamberland, Amy Tam, L. Michael Thomas, Grace Yan, Aaron M. D’ Antona, Laura Lin, Malgorzata Nocula-Lugowska, Yan Zhang, Eric Sousa, Justin Cohen, Ling Gu, Molica Abel, Jacob Donahue, Sean Lim, Caryl Meade, Jing Zhou, Logan Riegel, Alex Birch, Brian J. Fennell, Edward Franklin, Jose M. Gomes, Boriana Tzvetkova, John J. Scarcelli
Rok vydání: 2022
Předmět:
Zdroj: Journal of Biotechnology. 360:79-91
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2022.10.016
Popis: This study has employed mammalian transient expression systems to generate afucosylated antibodies and antibody Fc mutants for rapid candidate screening in discovery and early development. While chemical treatment with the fucose analogue 2-fluoro-peracetyl-fucose during transient expression only partially produced antibodies with afucosylated N-glycans, the genetic inactivation of the FUT8 gene in ExpiCHO-S™ by CRISPR/Cas9 enabled the transient production of fully afucosylated antibodies. Human IgG
Databáze: OpenAIRE
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