Commensal isolates of methicillin-resistant Staphylococcus epidermidis are also well equipped to produce biofilm on polystyrene surfaces
| Autor: | Agnes Marie Sá Figueiredo, Amada Zambrana Coronado, Lenise Arneiro Teixeira, Gabrielle Luck de Araujo, Camila Barbosa de Carvalho, Leonardo Rocchetto Coelho, Ronaldo Rozenbaum, Bernadete Teixeira Ferreira-Carvalho, Rafael Muniz Maciel |
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| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Micrococcaceae Operon Molecular Sequence Data Microbial Sensitivity Tests Bacterial Adhesion law.invention Bacterial genetics Microbiology law Staphylococcus epidermidis Humans Pharmacology (medical) Polymerase chain reaction Pharmacology Base Sequence biology Biofilm Methicillin-resistant Staphylococcus epidermidis biochemical phenomena metabolism and nutrition biology.organism_classification Anti-Bacterial Agents Infectious Diseases Genes Bacterial Biofilms Polystyrenes Methicillin Resistance Bacteria |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 57:855-864 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkl071 |
| Popis: | OBJECTIVES: To study biofilm production and to detect icaAD, atlE and aap genes in 137 isolates of methicillin-resistant Staphylococcus epidermidis (MRSE) obtained from healthy individuals from the community (35 isolates), from hospitalized patients at the Antonio Pedro University Hospital (25 isolates) and from individuals from a home-care system (HCS; 77 isolates). METHODS: Biofilm production was determined in vitro using polystyrene inert surfaces. icaAD, atlE and aap genes were detected using PCR. Hybridization experiments were also carried out to confirm some PCR results. Antimicrobial susceptibility testing was carried out using the NCCLS methods. RESULTS: Although many of the commensal MRSE isolates produced biofilms, the percentage of biofilm producers was significantly higher (P = 0.0107) among hospital isolates (76%) than among isolates from the community (60%) and from the HCS (57%). An association was observed between multiresistance and biofilm production for isolates obtained from healthy individuals from the community and from household contacts from the HCS (P < 0.0001). The concomitant presence of the ica operon and atlE and aap genes was associated with the strong biofilm-producer phenotype (P < 0.0001). CONCLUSION: Because many of the commensal MRSE isolates obtained from nares produced biofilms and carried icaAD, aap and atlE genes, biofilms or such genetic elements should not be used as markers for clinical significance. The biofilm environment seems to increase genetic exchanges and hence may contribute to multiresistance phenotypes. |
| Databáze: | OpenAIRE |
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