A new molecular method for rapid etiological diagnosis of sepsis with improved performance
| Autor: | Massimo Crapis, Francesco Michelangelo Turrini, Franca Mannu, Franco Carta, Manuela Avolio, Enrico Turrini, Lucia Pagani, Nilla Tosoni, Shamanta Grosso, Rita De Rosa |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty medicine.diagnostic_test business.industry Real-Time Polymerase Chain Reaction medicine.disease Microbiology Sepsis Improved performance Molecular microbiology Internal medicine Bacteremia Multiplex polymerase chain reaction medicine Etiology Humans Blood culture business Pathogen |
| Zdroj: | Future Microbiology. 16:741-751 |
| ISSN: | 1746-0921 1746-0913 |
| DOI: | 10.2217/fmb-2020-0154 |
| Popis: | The value of blood cultures for confirming the clinical diagnosis of sepsis is suboptimal. There is growing interest in the potential of real-time PCR technology by detection of minute amounts of pathogen DNA in patient blood samples with results available within 4–6 h. Adopting a two-step approach, we evaluated the compliance of two versions of the MicrobScan assay on a total of 748 patients with suspected bloodstream infections. The results obtained with a second version of the MicrobScan assay are characterized by increased specificity (from 95.1 to 98.2%) and sensitivity (from 76.7 to 85.1), increased throughput and the possibility of simultaneously testing different kinds of samples collected from the potential sites of infection and utilizing different syndromic panels. |
| Databáze: | OpenAIRE |
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