Voluntary Exercise Positively Affects the Recovery of Long-Nerve Gap Injury Following Tube-Bridging with Human Skeletal Muscle-Derived Stem Cell Transplantation
| Autor: | Ippei Yamato, Daisuke Maki, Tetsuro Tamaki, Hiroya Seta, Shuichi Soeda, Akihito Kazuno, Yoshiyasu Uchiyama, Nobuyuki Nakajima |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
p75
0301 basic medicine medicine.medical_specialty CD34 lcsh:Medicine sensory nerve function Article tetanic tension motor nerve function N200 myelin basic protein 03 medical and health sciences Myelin 0302 clinical medicine Internal medicine medicine Tibial nerve business.industry lcsh:R Skeletal muscle General Medicine Sciatic nerve injury Nerve injury medicine.disease 030104 developmental biology medicine.anatomical_structure Endocrinology Stem cell medicine.symptom business 030217 neurology & neurosurgery Blood vessel |
| Zdroj: | Journal of Clinical Medicine; Volume 7; Issue 4; Pages: 67 Journal of Clinical Medicine, Vol 7, Iss 4, p 67 (2018) Journal of Clinical Medicine |
| ISSN: | 2077-0383 |
| DOI: | 10.3390/jcm7040067 |
| Popis: | The therapeutic effects of voluntary exercise on the recovery of long-gap nerve injury following the bridging of an acellular conduit filled with human skeletal muscle-derived stem cells (Sk-SCs) have been described. Human Sk-SCs were sorted as CD34+/45− (Sk-34) cells, then cultured/expanded under optimal conditions for 2 weeks. Surgery to generate a long-gap sciatic nerve injury was performed in athymic nude mice, after which the mice were divided into exercise (E) and non-exercise (NE) groups. The mice were housed in standard individual cages, and voluntary exercise wheels were introduced to the cages of the E group one week after surgery. After 8 weeks, the human Sk-34 cells were actively engrafted, and showed differentiation into Schwann cells and perineurial cells, in both groups. The recovery in the number of axons and myelin in the conduit and downstream tibial nerve branches, and the lower hindlimb muscle mass and their tension output, was consistently higher by 15–25% in the E group. Moreover, a significantly higher innervation ratio of muscle spindles, reduced pathological muscle fiber area, and acceleration of blood vessel formation in the conduit were each observed in the E group. These results showed that the combined therapy of tube-bridging, Sk-34 cell transplantation, and voluntary exercise is a potentially practical approach for recovery following long-gap nerve injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|