IonWorks™ HT: A New High-Throughput Electrophysiology Measurement Platform
| Autor: | Derek J. Trezise, Jennings F. Worley, Brad Neagle, Kirk S. Schroeder |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
0301 basic medicine
Potassium Channels Computer science Voltage clamp Analytical chemistry CHO Cells 01 natural sciences Biochemistry Sodium Channels Analytical Chemistry 03 medical and health sciences Automated patch clamp Cricetinae Animals Humans Patch clamp Throughput (business) Ion channel Sodium channel Pipette 0104 chemical sciences Electrophysiology 010404 medicinal & biomolecular chemistry 030104 developmental biology Molecular Medicine Biological Assay Biotechnology Biomedical engineering |
| Zdroj: | SLAS Discovery. 8:50-64 |
| ISSN: | 2472-5552 |
| DOI: | 10.1177/1087057102239667 |
| Popis: | 3To address the throughput restrictions of classical patch clamp electrophysiology, Essen Instruments has developed a platebased electrophysiology measurement platform. The instrument is an integrated platform that consists of computer-controlled fluid handling, recording electronics, and processing tools capable of voltage clamp whole-cell recordings from thousands of individual cells per day. To establish a recording, the system uses a planar, multiwell substrate (a PatchPlate™). The system effectively positions 1 cell into a hole separating 2 fluid compartments in each well of the substrate. Voltage control and current recordings from the cell membrane are made subsequent to gaining access to the cell interior by applying a permeabilizing agent to the intracellular side. Based on the multiwell design of the PatchPlate™, voltage clamp recordings of up to 384 individual cells can be made in minutes and are comparable to measurements made using traditional electrophysiology techniques. An integrated pipetting system allows for up to 2 additions of modulation agents. Typical throughput, measurement fidelity, stability, and comparative pharmacology of a recombinant voltage-dependent sodium channel (hNav1.3) and a voltage-gated potassium channel (hKv1.5) exogenously expressed in CHO cells are presented. The IonWorks™ HT device can be used in biophysical and pharmacological profiling of ion channels in an environment compatible with high-capacity screening. (Journal of Biomolecular Screening 2003:50-64) |
| Databáze: | OpenAIRE |
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