Isolation and convulsivant effect of the 'insulin-like' protein obtained from the exocrine pancreas of Bradypus tridactylus L
| Autor: | R.G. Pessoa, A.M.F. Souza, H.B. Coutinho, R.L. Hunter, E.M.P. Freitas, F.J.C. Aguiar, L.M.P Ferreira |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
Insulin medicine.medical_treatment Convulsants Stimulation Radioimmunoassay General Medicine Biology Xenarthra Sloths Electrophoresis Basal (phylogenetics) Isoelectric point Pancreatic Juice Biochemistry Pancreatic juice Pi medicine Animals Electrophoresis Polyacrylamide Gel Female Isoelectric Focusing Pancreas |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Physiology. 74:951-954 |
| ISSN: | 0300-9629 |
| Popis: | 1. 1. A pancreatic insulin-like protein fraction with low electrophoretic mobility showing high molecular weight is present. 2. 2. Isoelectric focussing studies showed that the high molecular weight protein has a pI about 7.4 when used at a pH range between 3.5 and 8.0. 3. 3. The partially purified aldehyde-fuchsin positive high molecular weight protein fraction gave a positive effect for the convulsivant test in mice. 4. 4. A high molecular weight insulin-like protein in pancreatic juice was found. 5. 5. Insulin was found by radioimmunoassay in the basal and post stimulation pancreatic juice. |
| Databáze: | OpenAIRE |
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