An infectious cDNA copy of the genome of a non-cardiovirulent coxsackievirus B3 strain: its complete sequence analysis and comparison to the genomes of cardiovirulent coxsackieviruses
| Autor: | Charles J. Gauntt, Nora M. Chapman, S. Tracy, Z. Tu |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
DNA Complementary viruses Molecular Sequence Data Restriction Mapping Genome Viral Viral Nonstructural Proteins Biology Transfection Virus Replication Genome Virus Mice Complete sequence chemistry.chemical_compound Capsid Species Specificity Virology Complementary DNA medicine Animals Humans T7 RNA polymerase cardiovascular diseases Cloning Molecular DNA Primers Genetics Mice Inbred C3H Base Sequence Nucleic acid sequence virus diseases Heart General Medicine musculoskeletal system Enterovirus B Human chemistry Viral replication DNA Viral cardiovascular system DNA HeLa Cells medicine.drug |
| Zdroj: | Archives of Virology. 135:115-130 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/bf01309769 |
| Popis: | The genome of the non-cardiovirulent coxsackievirus B3 (CVB3) strain CVB3/0 was cloned and sequenced to aid in the elucidation of the viral genetic basis for the CVB3 cardiovirulent phenotype. Reverse-transcribed sub-genomic complementary DNA (cDNA) fragments were enzymatically amplified using generic oligonucleotide primers and were assembled as a complete infectious genomic copy (pCVB3-0) downstream of the T7 RNA polymerase promoter. Positive-strand viral RNA transcribed from pCVB3-0 using T7 RNA polymerase and transfected into HeLa cells produced infectious virus (CVB3/0c). No differences in phenotype were observed comparing growth of CVB3/0c to the parental CVB3/0 in HeLa single-step growth curves, virus yields, or plaque size. When inoculated into C3H/HeJ mice, CVB3/0c achieved cardiac titers equivalent to the parental CVB3/0 and like the parental virus, demonstrated a non-cardiovirulent phenotype. The nucleotide sequence of the cloned CVB3/0 genome was determined and compared to the genomes of infectious cDNA clones of cardiovirulent CVB3 strains. Two consistent differences among nucleotides in non-translated regions and 8 amino acid differences relative to two well-characterized infectious cDNA copies of genomes from cardiovirulent CVB3 strains were identified. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink