Characterization of GFR, a novel guanine nucleotide exchange factor for Rap1

Autor: Naoko Tajima, Yasutaka Hoshi, Yasunobu Kuraishi, Tamotsu Ichiba, Yoshikatsu Eto
Rok vydání: 1999
Předmět:
endocrine system
Molecular Sequence Data
Biophysics
Cell Cycle Proteins
Human brain
Kidney
Transfection
urologic and male genital diseases
Biochemistry
Cell Line
cdc25 domain
Nuclear localization signal
GTP-Binding Proteins
Structural Biology
Cyclic AMP
Genetics
Guanine Nucleotide Exchange Factors
Humans
Tissue Distribution
Amino Acid Sequence
RNA
Messenger
Northern blot
Molecular Biology
reproductive and urinary physiology
Cell Nucleus
Rap1
Messenger RNA
Sequence Homology
Amino Acid
ras-GRF1
urogenital system
Chemistry
HEK 293 cells
Brain
Proteins
Ras family GTPase
Cell Biology
Molecular biology
Guanine Nucleotides
enzymes and coenzymes (carbohydrates)
rap GTP-Binding Proteins
CAMP binding
ras Guanine Nucleotide Exchange Factors
Guanine nucleotide exchange factor
Nuclear localization sequence
HeLa Cells
Zdroj: FEBS Letters. 457:85-89
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(99)01012-1
Popis: Three groups of Rap1-specific guanine nucleotide exchange factors including C3G, CalDAG-GEFI, and Epac/cAMP-GEFI/II have been identified to date. In the present study, we report a new Rap1 guanine nucleotide exchange factor which we have named GFR (guanine nucleotide exchange factor for Rap1). GFR shows close sequence similarity to EPAC/cAMP-GEFI/II although GFR lacks a cAMP binding domain and contains a nuclear localization signal. We demonstrated that GFR can activate Rap1 but not H-Ras in 293T cells and that the cdc25 domain of GFR is required for the activation of Rap1. Northern blot analysis suggested that GFR mRNA is strongly expressed in the brain. In transfected HeLa cells, GFR has been found to be localized in the nuclei.
Databáze: OpenAIRE
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