Honey activity on the proliferation and migration of oral squamous cell carcinoma: In vitro and bioinformatic analysis
| Autor: | Anna Christina de Almeida, Juliana Rezende Sa Miranda Goncalves, Janainne Nunes Alves, Sabrina Ferreira de Jesus, Ricardo Jardim Neiva, Lucyana Conceiçao Farias, Alex Sander Rodrigues Cangussu, Hercules OtacÃlio Santos, Eliane Macedo Sobrinho Santos, Kattyanne Souza Costa, André Luiz Sena Guimarães, Igor Viana Brandi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Aroeira do sertão animal structures biology Mel de abelha -- Jequitinhonha Rio Vale (MG e BA) General Engineering food and beverages General Physics and Astronomy General Medicine biology.organism_classification Phenotype Mel -- Uso terapêutico General Biochemistry Genetics and Molecular Biology In vitro GeneCards Squamous carcinoma 03 medical and health sciences 030104 developmental biology Biochemistry Cell culture Polyphenol General Agricultural and Biological Sciences Gene Abelha-européia Myracrodruon urundeuva |
| Zdroj: | Repositório Institucional da UFMG Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
| ISSN: | 1992-2248 |
| Popis: | This study aimed to investigate, in oral squamous carcinoma cells (OSCC), the antineoplastic effect of Apis mellifera honey produced from Aroeira and wildflower, in the region of Jequitinhonha River Valley, Brazil. Furthermore, we investigate the molecular mechanism of honey performance through bioinformatic analysis. Honey and OSCC-related gene list were screened using GeneCards. The online tool STRING was used to identify the potential interaction networks of protein products of these genes. The genes with the highest weighted number of links (WNL) were called leader genes. GO and KEGG pathway analysis was performed to classify the genes. We use a search tool STITCH for interactions of chemicals-proteins. The chemical characterization of honey and its antiproliferative and antimigratory effects in SCC-9 cell lines was evaluated in vitro. The analysis of the interaction network obtained from the STRING database indicated by the following leader genes: TNF, IL6 and HDAC1. GO analysis for the honey, and OSCC-related proteins revealed that leader genes-regulated cancers genes were markedly enriched in some functions. Through the chemical characterization, the bioactive classes: steroids/triterpenoids, flavonoids, saponins, alkaloids, and tannins were identified. Polyphenols act in activating CASP3 and TP53 and inhibit the action of DNMT1. The honey produced from Aroeira and wildflower reduced proliferative phenotype and migration of OSCC cells. Key words: Aroeira honey, Myracrodruon urundeuva, wild honey, Apis mellifera, therapeutic, Jequitinhonha River Valley. |
| Databáze: | OpenAIRE |
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