Long-term administration of green tea catechins prevents age-related spatial learning and memory decline in C57BL/6 J mice by regulating hippocampal cyclic amp-response element binding protein signaling cascade
| Autor: | Qingjiang Li, Jianbo Wang, Yanmeng Li, Xinrong Pei, Haifeng Zhao, Z. Zhang, M.Y. Cai, Zhigang Liu |
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| Rok vydání: | 2009 |
| Předmět: |
Aging
medicine.medical_specialty Hippocampus Morris water navigation task Hippocampal formation CREB Antioxidants Catechin Mice Random Allocation Memory Neurotrophic factors Ca2+/calmodulin-dependent protein kinase Internal medicine medicine Animals Learning Phosphorylation Cyclic AMP Response Element-Binding Protein Maze Learning Brain-derived neurotrophic factor biology Chemistry Brain-Derived Neurotrophic Factor General Neuroscience Intracellular Signaling Peptides and Proteins Membrane Proteins Mice Inbred C57BL Endocrinology Proto-Oncogene Proteins c-bcl-2 Space Perception Synaptic plasticity biology.protein Female Calcium-Calmodulin-Dependent Protein Kinase Type 2 Disks Large Homolog 4 Protein Guanylate Kinases Neuroscience Signal Transduction |
| Zdroj: | Neuroscience. 159:1208-1215 |
| ISSN: | 0306-4522 |
| Popis: | Flavonoid-rich foods have been shown to be effective at reversing age-related deficits in learning and memory in both animals and humans. However, little investigation of the preventative effects of flavonoids on the naturally aged animals was reported. In our study, 14-month-old female C57BL/6 J mice were orally administered 0.025%, 0.05% and 0.1% green tea catechins (GTC, w/v) in drinking water for 6 months; we found that a supplementation with 0.05% or 0.1% GTC prevented age-related spatial learning and memory decline of mice in the Morris water maze. Better performance of GTC-treated mice was associated with increased levels of cAMP-response element binding protein (CREB) phosphorylation in the hippocampus. The expressions of brain-derived neurotrophic factor (BDNF) and Bcl-2, two target genes of CREB which can exhibit long-term regulatory roles in synaptic plasticity and synaptic structure, were also increased. We also found that long-term 0.05% or 0.1% GTC administration prevented age-related reductions of two representative post-synaptic density proteins PSD95 and Ca(2+)/calmodulin-dependent protein kinase II, suggesting that synaptic structural changes may be involved. These results demonstrated that long-term 0.05% or 0.1% green tea catechin administration may prevent age-related spatial learning and memory decline of female C57BL/6 J mice by regulating hippocampal CREB signaling cascade. |
| Databáze: | OpenAIRE |
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