Biophysical properties of salmonid alphaviruses: influence of temperature and pH on virus survival
| Autor: | K Cherry, Christopher J. Staples, David Graham, A Gordon, H M Rowley, H Jewhurst, C J Wilson |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
biology Veterinary (miscellaneous) Temperature Fresh Water Alphavirus Hydrogen-Ion Concentration Aquatic Science biology.organism_classification Virology Virus Persistence (computer science) chemistry Animals Virus Inactivation Seawater Organic matter Food science Alkaline hydrolysis Salmonidae Waste disposal |
| Zdroj: | Journal of Fish Diseases. 30:533-543 |
| ISSN: | 1365-2761 0140-7775 |
| DOI: | 10.1111/j.1365-2761.2007.00811.x |
| Popis: | A series of laboratory studies were undertaken to investigate the survival of salmonid alphaviruses (SAV) under a range of conditions relevant to waste disposal, persistence and spread in the field, and to laboratory studies and testing. SAV was found to be rapidly inactivated in the presence of high levels of organic matter at 60 degrees C at pH 7.2 and at pH 4 and pH 12 at 4 degrees C, suggesting that composting, ensiling and alkaline hydrolysis would all be effective at inactivating virus in fish waste. Testing was conducted under sterile conditions at 4, 10, 15 and 20 degrees C in sea water, half-strength sea water and fresh (hard) water, both in the absence and the presence of added organic matter. Virus survival was shown to be inversely related to temperature, and to be reduced by the presence of organic matter. Calculated half lives (t(1/2)) under these conditions ranged from 61.0 to 1.5 days. Testing in non-sterile sea water resulted in reduced t(1/2) values. The half life of SAV in serum was also found to be inversely related to temperature, emphasizing the need for rapid shipment of samples at 4 degrees C to laboratories for virus isolation studies. |
| Databáze: | OpenAIRE |
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