Vital marker for muscle nuclei in myoblast transfer
| Autor: | Tena G. Goodwin, Hua-ju Li, Ming Chen, Peter K. Law, Q. Fang |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Stilbamidines Physiology Muscle Proteins Biology Fluorescence Mice Physiology (medical) Labelling medicine Myocyte Animals Humans Fluorescent Dyes Pharmacology Cell Nucleus Muscles General Medicine In vitro incubation musculoskeletal system Molecular biology Mice Inbred C57BL medicine.anatomical_structure Vital stain Subculture (biology) Nucleus |
| Zdroj: | Canadian journal of physiology and pharmacology. 69(1) |
| ISSN: | 0008-4212 |
| Popis: | A new method is developed using Fluoro-Gold (FG) as a vital stain to label the nuclei of donor myoblasts in myoblast transfer studies. In vitro incubation with 0.01% FG for 16 h resulted in 100% nuclei labelling. Intensive fluorescence persisted following 9 days of subculture, when the human myoblasts were injected into the quadriceps of mouse recipients immunosuppressed with cyclosporine. Injected muscles showed mosaicism of host and donor nuclei 25 days after injection, indicating (i) survival and fusion among donor myoblasts, and (ii) fusion between host and donor cells. FG labelling was not observed in control muscles injected with an equal volume of FG-labelled dead myoblasts, 0.01% FG medium, or phosphate-buffered saline.Key words: Fluoro-Gold, nucleus vital stain, myoblast culture and transplant. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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