Identification and molecular characterization of Echinococcus canadensis G6/7 in dogs from Corsica, France

Autor: Sébastien Grech-Angelini, Céline Richomme, Carine Peytavin de Garam, Jean-Marc Boucher, Frédéric Grenouillet, Oscar Maestrini, Gérald Umhang, François Casabianca, Franck Boué
Přispěvatelé: Laboratoire de Recherches sur le Développement de l'Elevage (LRDE), Institut National de la Recherche Agronomique (INRA), Laboratoire de la rage et de la faune sauvage de Nancy (LRFSN), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Laboratoire Chrono-environnement - UFC (UMR 6249) (LCE), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Centre Hospitalier Régional Universitaire [Besançon] (CHRU Besançon), Unité de recherche Développement de l'Elevage (LRDE)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Genotype
Swine
Echinococcus canadensis G6/7
030231 tropical medicine
Population
Sus scrofa
Zoology
Corsica
Biology
03 medical and health sciences
Feces
0302 clinical medicine
Dogs
Sensu
Wild boar
Echinococcosis
biology.animal
Surveys and Questionnaires
parasitic diseases
Prevalence
Dog
Helminths
Animals
Humans
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Echinococcus granulosus
education
030304 developmental biology
0303 health sciences
education.field_of_study
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
General Veterinary
Deworming
Haplotype
General Medicine
biology.organism_classification
Cystic echinococcosis
Infectious Diseases
Genes
Mitochondrial
Parasitology
Haplotypes
Insect Science
Cyclooxygenase 1
France
Oxidoreductases
Abattoirs
Zdroj: Parasitology Research
Parasitology Research, Springer Verlag (Germany), 2019, 118 (4), pp.1313-1319. ⟨10.1007/s00436-019-06261-6⟩
ISSN: 0932-0113
1432-1955
Popis: International audience; Recent surveys at slaughterhouses confirmed the presence of three different species of Echinococcus granulosus sensu lato in France: E. granulosus sensu stricto, E. ortleppi, and E. canadensis G6/7. The latter species was only identified on the French Mediterranean island of Corsica, with a high prevalence in pigs and wild boar. In order to investigate the life cycle of E. canadensis in this region, dog feces were collected in 31 municipalities, mainly from individual kennels. The analysis of fecal samples from 259 dogs by multiplex real-time PCR shows no infection by E. granulosus sensu stricto, but three dogs were infected by E. canadensis G6/7. Genetic analyses of mitochondrial genes (cox1, nad1, nad3, atp6) revealed in two dogs a haplotype previously identified in pigs. The third dog was infected by a new haplotype differing only from the two others from dogs by two mutations in the nad3 gene. This latter haplotype is genetically closer to those identified in pigs rather than those from wild boars. Analysis of questionnaires completed by the owners revealed that the sampled dog population was almost exclusively composed of hunting dogs that had been infrequently dewormed. Most of the owners (78%) leave carcasses of hunter-harvested wild boar in close proximity to their dogs. Nevertheless, genetic results seem to indicate that the three dogs were infected due to their consumption of a pig's infected viscera following home slaughtering. This study confirms the role of dogs as definitive hosts of E. canadensis G6/7 in Corsica. Further molecular studies, notably in human cases, are needed to assess the zoonotic impact of E. canadensis G6/7 in this region.
Databáze: OpenAIRE
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