Cloning and Expression of Rat Prostaglandin Endoperoxide Synthase (Cyclooxygenase)-2 cDNA
| Autor: | S. A. Culp, Wanda Cromlish, Chi-Chung Chan, Brian P. Kennedy |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Lipopolysaccharides
Male DNA Complementary Lipopolysaccharide Molecular Sequence Data Biophysics Gene Expression Prostaglandin Biology Carrageenan Transfection Polymerase Chain Reaction Biochemistry Dinoprostone Cell Line Rats Sprague-Dawley Mice chemistry.chemical_compound Complementary DNA Gene expression Animals Edema Humans Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Inflammation Messenger RNA Osteoblasts Base Sequence Sequence Homology Amino Acid Cell Biology Blotting Northern Molecular biology Rats chemistry Prostaglandin-Endoperoxide Synthases Enzyme Induction biology.protein Arachidonic acid Cyclooxygenase |
| Zdroj: | Biochemical and Biophysical Research Communications. 197:494-500 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1993.2506 |
| Popis: | Rat prostaglandin endoperoxidase synthase-2 (PGHS-2) cDNA was cloned from rat calvarial osteoblasts total RNA by RT-PCR. The primary sequence of rat PGHS-2 had 98% and 92% identity to the mouse and human enzymes, respectively. Transfection of the rat PGHS-2 cDNA into COS 7 cells, followed by the addition of 20 microM arachidonic acid, resulted in a dramatic increase in PGE2 released from these cells. The amount of PGE2 produced was comparable to that obtained from cells similarly transfected with human PGHS-1 cDNA. In the rat paw carrageenin-oedema inflammatory model, the injected paw had elevated levels of PGHS-2 mRNA compared to the control paw. In a rat pyrexia model, injection of the pyrogen lipopolysaccharide, resulted in elevated levels of PGHS-2 mRNA in the brain. These results suggest that PGHS-2 expression plays a role both in inflammation and fever. |
| Databáze: | OpenAIRE |
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