Low prevalence of the BCR–ABL1 fusion gene in a normal population in southern Sarawak
| Autor: | Isabel Lim Fong, Siow Phing Tay, Lela Su'ut, Motomi Osato, Goro Sashida, Anselm Ting Su, Jew Win Kuan, Sho Kubota |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
ABL Hematology Malaysia Myeloid leukemia BCR/ABL1 Fusion Gene Biology Asymptomatic Molecular biology Fusion gene Real-time polymerase chain reaction hemic and lymphatic diseases Internal medicine Proto-Oncogene Proteins c-bcr Prevalence medicine Humans Gene Fusion medicine.symptom Proto-Oncogene Proteins c-abl Gene |
| Zdroj: | International Journal of Hematology. 111:217-224 |
| ISSN: | 1865-3774 0925-5710 |
| DOI: | 10.1007/s12185-019-02768-x |
| Popis: | The BCR–ABL1 fusion gene is the driver mutation of Philadelphia chromosome-positive chronic myeloid leukemia (CML). Its expression level in CML patients is monitored by a real-time quantitative polymerase chain reaction defined by the International Scale (qPCRIS). BCR–ABL1 has also been found in asymptomatic normal individuals using a non-qPCRIS method. In the present study, we examined the prevalence of BCR–ABL1 in a normal population in southern Sarawak by performing qPCRIS for BCR–ABL1 with ABL1 as an internal control on total white blood cells, using an unbiased sampling method. While 146 of 190 (76.8%) or 102 of 190 (53.7%) samples showed sufficient amplification of the ABL1 gene at > 20,000 or > 100,000 copy numbers, respectively, in qPCRIS, one of the 190 samples showed amplification of BCR–ABL1 with positive qPCRIS of 0.0023% and 0.0032% in two independent experiments, the sequence of which was the BCR–ABL1 e13a2 transcript. Thus, we herein demonstrated that the BCR–ABL1 fusion gene is expected to be present in approximately 0.5–1% of normal individuals in southern Sarawak. |
| Databáze: | OpenAIRE |
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