Novel amplification unit at chromosome 3q25-q27 in human prostate cancer
| Autor: | Axel Bex, Thomas Zwergel, Volker Rohde, Michael Stoeckle, Margitta Retz, Bernd Wullich, Emmanuel Zimmer, H.-P. Sattler, Eckhart Meese, Ramona Lensch, Helmut Bonkhoff |
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| Rok vydání: | 2000 |
| Předmět: |
Genetics
Male Urology Gene Amplification Cancer Nucleic Acid Hybridization Prostatic Neoplasms Chromoplexy Biology medicine.disease Polymerase Chain Reaction Prostate cancer Blotting Southern Oncology DU145 Gene duplication medicine Cancer research Disease Progression Humans Genes Tumor Suppressor Chromosomes Human Pair 3 Gene Southern blot Comparative genomic hybridization |
| Zdroj: | The Prostate. 45(3) |
| ISSN: | 0270-4137 |
| Popis: | BACKGROUND In prostate carcinoma, amplification of the genes c-MYC, Her2/NEU, and the androgen receptor gene has been documented, with gene amplification being related to progressive tumor growth. Recently, using comparative genomic hybridization (CGH), we provided evidence for DNA copy number gains at chromosome 3q25–q26 in prostate cancer [Sattler et al.: Prostate 39:79–86, 1999]. METHODS In this study, additional prostatic tumors were evaluated by CGH to determine the frequency of DNA overrepresentation at 3q. Comparative PCR and Southern blot analyses were applied to determine whether known genes are involved in DNA copy number gains. RESULTS By CGH, DNA copy number gains, all of which involved chromosome region 3q25–q26, were disclosed in 50% of the prostate tumors analyzed. There was no evidence for high-level amplification. The analysis of 12 genes from 3q25–q27 by comparative PCR revealed amplification in 6 (35.3%) of 17 tumors tested. Amplification was detected for the genes IL12A, MDS1, SLC2A2, and SOX2, with coamplification of three genes in two tumors. IL12A was amplified as single gene in three tumors and in a subline of the DU145 cell line, SLC2A2 in one tumor. CONCLUSIONS Our studies revealed a novel amplification unit at 3q25–q27 in prostate carcinoma, with the genes IL12A, MDS1, SLC2A2, and SOX2 being located within the amplification unit. A common region of amplification was evident spanning the IL12A gene locus at 3q25–q26.2. Possibly, IL12A indicates an adjacent, till now unidentified gene which is important in the development of prostate cancer. Prostate 45:207–215, 2000. © 2000 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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