CrMAPK3 regulates the expression of iron-deficiency-responsive genes in Chlamydomonas reinhardtii
| Autor: | Xiaowen Fei, Junmei Yu, Yajun Li, Xiaodong Deng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:Animal biochemistry Chlamydomonas reinhardtii Chimeric gene Bioinformatics Genes Plant 01 natural sciences Biochemistry lcsh:Biochemistry 03 medical and health sciences RNA interference Gene Expression Regulation Plant Stress Physiological Arabidopsis Gene expression Gene family lcsh:QD415-436 Gene Silencing RNA Messenger Mitogen-activated protein kinases Molecular Biology Gene lcsh:QP501-801 Mitogen-Activated Protein Kinase 3 biology Iron deficiency Chlamydomonas Iron Deficiencies biology.organism_classification Cell biology 030104 developmental biology 010606 plant biology & botany Research Article Signal Transduction Real-time PCR |
| Zdroj: | BMC Biochemistry, Vol 18, Iss 1, Pp 1-10 (2017) BMC Biochemistry |
| ISSN: | 1471-2091 |
| DOI: | 10.1186/s12858-017-0081-5 |
| Popis: | Under iron-deficient conditions, Chlamydomonas exhibits high affinity for iron absorption. Nevertheless, the response, transmission, and regulation of downstream gene expression in algae cells have not to be investigated. Considering that the MAPK pathway is essential for abiotic stress responses, we determined whether this pathway is involved in iron deficiency signal transduction in Chlamydomonas. Arabidopsis MAPK gene sequences were used as entry data to search for homologous genes in Chlamydomonas reinhardtii genome database to investigate the functions of mitogen-activated protein kinase (MAPK) gene family in C. reinhardtii under iron-free conditions. Results revealed 16 C. reinhardtii MAPK genes labeled CrMAPK2–CrMAPK17 with TXY conserved domains and low homology to MAPK in yeast, Arabidopsis, and humans. The expression levels of these genes were then analyzed through qRT-PCR and exposure to high salt (150 mM NaCl), low nitrogen, or iron-free conditions. The expression levels of these genes were also subjected to adverse stress conditions. The mRNA levels of CrMAPK2, CrMAPK3, CrMAPK4, CrMAPK5, CrMAPK6, CrMAPK8, CrMAPK9, and CrMAPK11 were remarkably upregulated under iron-deficient stress. The increase in CrMAPK3 expression was 43-fold greater than that in the control. An RNA interference vector was constructed and transformed into C. reinhardtii 2A38, an algal strain with an exogenous FOX1:ARS chimeric gene, to silence CrMAPK3. After this gene was silenced, the mRNA levels and ARS activities of FOX1:ARS chimeric gene and endogenous CrFOX1 were decreased. The mRNA levels of iron-responsive genes, such as CrNRAMP2, CrATX1, CrFTR1, and CrFEA1, were also remarkably reduced. CrMAPK3 regulates the expression of iron-deficiency-responsive genes in C. reinhardtii. |
| Databáze: | OpenAIRE |
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