Continuous intravenous infusion in the unrestrained rat — procedures and results
| Autor: | I. C. Enninga, D. A. Cave, A. C. M. Schoenmakers, J. C. M. van der Hoeven, H. J. Van Wijk |
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| Rok vydání: | 1995 |
| Předmět: |
Male
0301 basic medicine Vena Cava Superior Health Toxicology and Mutagenesis Urinary system Femoral vein Urination Vena Cava Inferior Toxicology medicine.disease_cause Rats Sprague-Dawley Eating 03 medical and health sciences Catheters Indwelling 0302 clinical medicine Catheterization Peripheral Animals Medicine Infusions Intravenous Adverse effect Saline Solution Hypertonic Blood Cells 030102 biochemistry & molecular biology business.industry Body Weight Osmolar Concentration General Medicine Femoral Vein Cannula Rats Specific Pathogen-Free Organisms Sprague dawley 030220 oncology & carcinogenesis Anesthesia Toxicity Female Irritation business Perfusion Blood Chemical Analysis |
| Zdroj: | Human & Experimental Toxicology. 14:192-200 |
| ISSN: | 1477-0903 0960-3271 |
| Popis: | 1 A method of continuous infusion in the unrestrained rat is described, which provides a scientifically accept able and easily maintained rodent model for use in toxicological investigations. 2 Sprague Dawley SPF rats had cannulas implanted into the vena cava via the femoral vein, and were continu ously infused with physiological saline for a total of 28 or 90 days. 3 The results indicate that there was no change in body weight, food consumption, clinical observations or clinical biochemistry of infused rats when compared to non-infused rats. There were small changes in haema tological parameters, however none were toxicological ly significant. Urinary volume was increased and uri nary specific gravity and osmolality were decreased. At macroscopic and microscopic examination there were findings of scar formation associated with the area of surgery and minimal irritation in the area of the vena cava which accommodated the cannula. 4 These results indicate that implantation of a cannula into the vena cava of a rat and subsequent continuous intravenous infusion of physiological saline produces no toxicological adverse effects over a period of 90 days. Consequently, this model can be recommended for the continuous intravenous administration of test substances to rats. |
| Databáze: | OpenAIRE |
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