Comparison of Parasitological, Serological, and Molecular Tests for Visceral Leishmaniasis in HIV-Infected Patients: A Cross-Sectional Delayed-Type Study
| Autor: | Andrea Laender Pessoa de Mendonça, Marcos Roberto de Sousa, Edward Oliveira, Gláucia Fernandes Cota, Ana Rabello, Tália Santana Machado de Assis, Bruna Fernandes Pinto, Juliana Wilke Saliba, Betânia Mara de Freitas Nogueira, Luciana Inácia Gomes |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male medicine.medical_specialty Antibodies Protozoan HIV Infections Biology Real-Time Polymerase Chain Reaction Sensitivity and Specificity Corrections Gastroenterology Serology Agglutination Tests Virology Direct agglutination test Internal medicine parasitic diseases medicine Humans Fluorescent Antibody Technique Indirect Direct fluorescent antibody Leishmania Coinfection Leishmaniasis Articles Dipstick DNA Protozoan Middle Aged medicine.disease Cross-Sectional Studies Infectious Diseases Visceral leishmaniasis Real-time polymerase chain reaction Immunology Leishmaniasis Visceral Female Parasitology |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.13-0239 |
| Popis: | The aim of this study was to evaluate the accuracy of invasive and non-invasive tests for diagnosis of visceral leishmaniasis (VL) in a large series of human immunodeficiency virus (HIV)-infected patients. In this delayed-type cross-sectional study, 113 HIV-infected symptomatic patients were evaluated by an adjudication committee after clinical follow-up to establish the presence or absence of VL as the target condition (reference test). The index tests were recombinant K39 antigen-based immunochromatographic test (rK39), indirect fluorescent antibody test (IFAT), prototype kit of direct agglutination test (DAT-LPC), and real-time polymerase chain reaction (qPCR) in peripheral blood. Compared with parasitological test and adjudication committee diagnosis or latent class model analyses, IFAT and rk39 dipstick test presented the lowest sensitivity. DAT-LPC exhibited good overall performance, and there was no statistical difference between DAT-LPC and qPCR diagnosis accuracy. Real-time PCR emerges as a less invasive alternative to parasitological examination for confirmation of cases not identified by DAT. |
| Databáze: | OpenAIRE |
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