Relative gene expression of fatty acid synthesis genes at 60 days postpartum in bovine mammary epithelial cells of Surti and Jafarabadi buffaloes
| Autor: | Balkrishna Brahmkshtri, Mamta Janmeda, V. B. Kharadi, K. K. Tyagi, Umed V. Ramani, Gaurav Pandya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Veterinary medicine Surti Biology SF1-1100 Andrology 03 medical and health sciences chemistry.chemical_compound Butyrophilin Lactation Gene expression SF600-1100 medicine Gene Fatty acid synthesis buffalo ACACA Lipoprotein lipase milk General Veterinary 0402 animal and dairy science food and beverages Jafarabadi 04 agricultural and veterinary sciences 040201 dairy & animal science Breed Animal culture 030104 developmental biology medicine.anatomical_structure chemistry gene expression Research Article |
| Zdroj: | Veterinary World, Vol 10, Iss 5, Pp 467-476 (2017) Veterinary World |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Aim Aim of the study was to study the relative gene expression of genes associated with fatty acid synthesis at 60 days postpartum (pp) in bovine mammary epithelial cells (MECs) of Surti and Jafarabadi buffaloes. Materials and methods A total of 10 healthy Surti and Jafarabadi buffaloes of each breed were selected at random from Livestock Research Station, Navsari and Cattle Breeding Farm, Junagadh, Gujarat, respectively, for this study. Milk sample was collected from each selected buffalo at day 60 pp from these two breeds to study relative gene expression of major milk fat genes using non-invasive approach of obtaining primary bovine MECs (pBMEC) from milk samples. Results In this study overall, the relative expression of the six major milk lipogenic genes butyrophilin subfamily 1 member A1 (BTN1A1), stearoyl-CoA desaturase (SCD), lipoprotein lipase (LPL), glycerol-3-phosphate acyltransferase mitochondrial (GPAM), acetyl-coenzyme A carboxylase alpha (ACACA), and lipin (LPIN) did not show changes in expression patterns at 60th day of lactation in both Surti and Jafarabadi buffaloes. Conclusion The pBMEC can be successfully recovered from 1500 ml of milk of Surti and Jafarabadi buffaloes using antibody-mediated magnetic bead separation and can be further used for recovering RNA for down step quantification of major milk lipogenic gene expression. The relative expression of the six major milk lipogenic genes BTN1A1, SCD, LPL, GPAM, ACACA, and LPIN did not show changes in expression patterns in both Surti and Jafarabadi buffaloes, suggesting expression levels of lipogenic genes are maintained almost uniform till peak lactation without any significant difference. |
| Databáze: | OpenAIRE |
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