Colorimetric Chemosensor and Turn on Fluorescence Probe for pH Monitoring Based on Xanthene Dye Derivatives and its Bioimaging of Living Escherichia coli Bacteria
| Autor: | Ahmed H. Bedair, Shimaa T. Omara, Elkhabiry Shaban, Omaima M. Kandil, Mahmoud Basseem I. Mohamed, Tarek Aysha, Taha M. Elmorsi, Mervat El-Sedik |
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| Rok vydání: | 2019 |
| Předmět: |
Sociology and Political Science
Clinical Biochemistry Quantum yield Biosensing Techniques 010402 general chemistry medicine.disease_cause 01 natural sciences Biochemistry Turn (biochemistry) chemistry.chemical_compound Fluorescence microscope medicine Escherichia coli Fluorescein Spectroscopy Fluorescent Dyes Molecular Structure 010405 organic chemistry Chemistry Optical Imaging Hydrogen-Ion Concentration Fluorescence 0104 chemical sciences Clinical Psychology Xanthenes Titration Colorimetry Law Social Sciences (miscellaneous) Nuclear chemistry |
| Zdroj: | Journal of fluorescence. 30(3) |
| ISSN: | 1573-4994 |
| Popis: | A new turn on fluorescence probe based on 3′,6′-dihydroxy-6-methyl-2-((pyridin-2-ylmethylene)amino)-4-(p-tolyl)spiro[benzo[f]isoindole-1,9′-xanthen]-3(2H)-one (BFFPH) derived from benzo[f]fluorescein was prepared. Full characterization of the prepared probe using spectroscopic analysis was described such as IR, NMR and MS spectra. The sensitivity of BFFPH for monitoring of pH change in alkaline medium was studied. BFFPH exhibited a high sensitivity to alkaline pH by two pKa values at 8.82 and 10.66 in UV/vis spectroscopy titration. The pH monitoring was studied in broad range of pH values (2.5–12.2) at two pKa values at 8.72 and 10.73 by recording the effect of pH on the fluorescence intensity of BFFPH. The acid-base reversibility character of the probe was investigated as well as the effect of the pH change on the fluorescence quantum yield. The application of the prepared BFFPH probe for detection of living Escherichia coli (E. coli) bacteria using confocal fluorescence microscope was investigated. |
| Databáze: | OpenAIRE |
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