Luminescent Amphiphilic Aminoglycoside Probes to Study Transfection
| Autor: | Johannes Koch, Micha Fridman, Qais Z. Jaber, Cecilia Vallet, Alexander Zimmermann, Steffen Riebe, Shirley K. Knauer, Kfir B. Steinbuch, Jens Voskuhl, Matthias Hayduk, Kateryna Loza |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
aggregation-induced emission
Cell Survival Static Electricity Chemie 010402 general chemistry Transfection 01 natural sciences Biochemistry Green fluorescent protein HeLa chemistry.chemical_compound Amphiphile Animals Humans bioimaging Molecular Biology Microscopy Confocal biology 010405 organic chemistry Chemistry Communication Organic Chemistry Cationic polymerization self-assembly transfection agents biology.organism_classification Lipids Communications 0104 chemical sciences Aminoglycosides HEK293 Cells Reagent cationic amphiphiles Biophysics Luminophore Tobramycin Molecular Medicine Self-assembly Biologie HeLa Cells Plasmids |
| Zdroj: | Chembiochem |
| ISSN: | 1439-7633 1439-4227 |
| Popis: | We report the characterization of amphiphilic aminoglycoside conjugates containing luminophores with aggregation‐induced emission properties as transfection reagents. These inherently luminescent transfection vectors are capable of binding plasmid DNA through electrostatic interactions; this binding results in an emission “on” signal due to restriction of intramolecular motion of the luminophore core. The luminescent cationic amphiphiles effectively transferred plasmid DNA into mammalian cells (HeLa, HEK 293T), as proven by expression of a red fluorescent protein marker. The morphologies of the aggregates were investigated by microscopy as well as ζ‐potential and dynamic light‐scattering measurements. The transfection efficiencies using luminescent cationic amphiphiles were similar to that of the gold‐standard transfection reagent Lipofectamine® 2000. Transfection tracker: Conjugates of luminophores with aggregation‐induced emission properties linked to the aminoglycoside tobramycin are reported. These compounds reveal an emission “on” behavior upon plasmid‐DNA binding and are able to transfect different mammalian cell lines. The cellular uptake was investigated using the emission properties of the formed plasmid DNA‐vector assemblies. |
| Databáze: | OpenAIRE |
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