Immunohistochemical Detection of Phosphatidylserine and Thrombospondin on Denucleating Erythroblasts in Rat Bone Marrow
| Autor: | Takanori Miki, Katsuhiko Warita, Yoshiki Takeuchi, Yoshiki Matsumoto, Nobuhiko Hoshi, Takahiko Etoh, Wang-Mei Qi, Eiichiro Takahara, Toshifumi Yokoyama, Kankanam Gamage Sanath Udayanga, Hiroshi Kitagawa |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Erythroblasts Apoptosis Phosphatidylserines Rat Bone Marrow Cell membrane chemistry.chemical_compound Apoptotic Process hemic and lymphatic diseases medicine Animals Erythropoiesis Rats Wistar Thrombospondin General Veterinary hemic and immune systems Phosphatidylserine Immunohistochemistry Rats Cell biology medicine.anatomical_structure chemistry Thrombospondins circulatory and respiratory physiology |
| Zdroj: | Journal of Veterinary Medical Science. 73:949-952 |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | The hypothesis that apoptotic factors play some roles in the denucleation of erythroblasts has been confirmed by the immunohistological detection of both phosphatidylserine and thrombospondin as phagocytosis-inducing factors in general apoptotic events. Both phosphatidylserine and thrombospondin were detected on the surface of cell membrane of mature erythroblasts, while thrombospondin was also detected in more immature erythroblasts. The intensities of their immune reactions increased as the erythroids matured. During denucleation, the positivities of both phosphatidylserine and thrombospondin were restricted on the surface of the cell membrane surrounding the protruding nuclei. Thus, the apoptotic process involves denucleation of erythroblasts and phosphatidylserine, and thrombospondin acts as phagocytosis-inducing factors in the denucleation event. |
| Databáze: | OpenAIRE |
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