Successful sperm cryopreservation in Egyptian spiny mice Acomys cahirinus
| Autor: | Sally Catt, Peter Temple-Smith, Jarrod McKenna, Mulyoto Pangestu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
media_common.quotation_subject Motility Reproductive technology Biology Cryopreservation law.invention Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Cryoprotective Agents law Genetics Animals Acrosome Molecular Biology 030304 developmental biology media_common Membrane Potential Mitochondrial 0303 health sciences 030219 obstetrics & reproductive medicine Extender biology.organism_classification Sperm Spermatozoa Semen Analysis Reproductive Medicine Spiny mouse Sperm Motility Animal Science and Zoology Murinae Reproduction Developmental Biology Biotechnology Semen Preservation |
| Zdroj: | Reproduction, fertility, and development. 32(16) |
| ISSN: | 1031-3613 |
| Popis: | The menstruating Egyptian spiny mouse has recently been proposed as a new animal model for reproductive health research. Unfortunately, little is known about reproduction in males. This study compared several characteristics of sperm function before and after cryopreservation. Epididymal spermatozoa were cryopreserved in different concentrations of raffinose and skim milk and tested for motility and membrane integrity (Experiment 1). Further evaluations of motility, plasma membrane and acrosome integrity, mitochondrial membrane potential and DNA integrity were conducted with the addition of l-glutamine to the extender (Experiment 2). The results show that, following cryopreservation, motility and membrane integrity were reduced, but were better maintained in the presence of l-glutamine (P |
| Databáze: | OpenAIRE |
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