Biological Glucose Metabolism Regulated Peptide Self-Assembly as a Simple Visual Biosensor for Glucose Detection
| Autor: | Xiao-Ding Xu, Si-Xue Cheng, Xian-Zheng Zhang, Jun Feng, Bing-Bing Lin, Ya Wang, Ren-Xi Zhuo |
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| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Polymers and Plastics biology Chemistry Organic Chemistry Lysine Peptide Biosensing Techniques Carbohydrate metabolism Enzymes Immobilized Gluconates Glucose Oxidase chemistry.chemical_compound Glucose Enzyme Biochemistry Aspartic acid Materials Chemistry Gluconic acid biology.protein Humans Glucose oxidase Peptides Oxidation-Reduction Biosensor |
| Zdroj: | Macromolecular Rapid Communications. 33:426-431 |
| ISSN: | 1022-1336 |
| DOI: | 10.1002/marc.201100689 |
| Popis: | A glucose oxidase (GOx)-mediated glucose metabolism was in vitro mimicked and employed to regulate the self-assembly of peptide-based building blocks. In this new stimuli-responsive self-assembly system, two peptide-based building blocks, respectively, having aspartic acid (gelator 1) and lysine (gelator 2) residues were designed and prepared. When adding glucose and GOx to the aqueous solution of gelator 1 or the self-assembled fibrillar hydrogel of gelator 2 to construct glucose metabolism system, the metabolic product (gluconic acid) can trigger the protonation of the peptide molecules and induce the phase transitions of gelators 1 (sol-gel) and 2 (gel-sol). Because this glucose metabolism regulated peptide self-assembly is built on the oxidation of glucose, it can be used as a simple visual biosensor for glucose detection. |
| Databáze: | OpenAIRE |
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