EPR Technology as Sensitive Method for Oxidative Stress Detection in Primary and Secondary Keratinocytes Induced by Two Selected Nanoparticles
| Autor: | Marcelo Calderón, A. Mensch, S. Grether-Beck, Silke B. Lohan, Martina C. Meinke, D. Höppe, Michael Giulbudagian, S. Ahlberg, J. Krutmann, Juergen Lademann |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Keratinocytes Cell Survival Biophysics Analytical chemistry 02 engineering and technology medicine.disease_cause Biochemistry Redox law.invention 03 medical and health sciences law medicine Humans Viability assay Electron paramagnetic resonance Cytotoxicity Incubation Cells Cultured Vehicle Emissions chemistry.chemical_classification Reactive oxygen species Electron Spin Resonance Spectroscopy Cell Biology General Medicine 021001 nanoscience & nanotechnology HaCaT Oxidative Stress 030104 developmental biology chemistry Nanoparticles 0210 nano-technology Reactive Oxygen Species Oxidative stress |
| Zdroj: | Cell biochemistry and biophysics. 75(3-4) |
| ISSN: | 1559-0283 |
| Popis: | Exogenous factors can cause an imbalance in the redox state of biological systems, promoting the development of oxidative stress, especially reactive oxygen species (ROS). To monitor the intensity of ROS production in secondary keratinocytes (HaCaT) by diesel exhaust particles and thermoresponsive nanogels (tNG), electron paramagnetic resonance (EPR) spectroscopy after 1 and 24 h of incubation, respectively, was applied. Their cytotoxicity was analyzed by a cell viability assay (XTT). For tNG an increase in the cell viability and ROS production of 10% was visible after 24 h, whereas 1 h showed no effect. A ten times lower concentration of diesel exhaust particles exhibited no significant toxic effects on HaCaT cells for both incubation times, thus normal adult human keratinocytes (NHK) were additionally analyzed by XTT and EPR spectroscopy. Here, after 24 h a slight increase of 18% in metabolic activity was observed. However, this effect could not be explained by the ROS formation. A slight increase in the ROS production was only visible after 1 h of incubation time for HaCaT (9%) and NHK (14%). |
| Databáze: | OpenAIRE |
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