Rapid analysis of neomycin in cochlear perilymph of guinea pigs using disposable SPE cartridges and high performance liquid chromatography-tandem mass spectrometry
| Autor: | Shili Zhang, Yan Yan, Hui Zhao, Mian Zu, Yuan Shuolong, Jia-Wei Han, Shi-Ming Yang, Shiwei Qiu, Yue Zhang, Jinan Jiang, Weiwei Guo |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Analyte
Clinical Biochemistry Guinea Pigs Perilymph Tandem mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry Matrix (chemical analysis) 03 medical and health sciences 0302 clinical medicine Limit of Detection Tandem Mass Spectrometry medicine Tobramycin Animals Chromatography High Pressure Liquid Chromatography Chemistry 010401 analytical chemistry Aminoglycoside Solid Phase Extraction Reproducibility of Results Neomycin Cell Biology General Medicine 0104 chemical sciences Round Window Ear Linear Models medicine.drug |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. |
| ISSN: | 1873-376X |
| Popis: | Irreversible hearing loss induced by aminoglycoside in human through local or systemic administration route negatively impacts quality of life. The aim of this work was to develop and validate an analytical method suitable for the detection and quantification of neomycin in cochlear perilymph of guinea pig after local application. The SupelMIP SPE column was used for the pre-treatment of matrix. Chromatographic separation was conducted by a reversed phase ODS column (100 × 2.1 mm, 3 μm) at 40 °C in gradient mode with 0.2‰ (v/v) HFBA in water and 0.2‰ (v/v) HFBA in acetonitrile as mobile phase, at a flow rate of 0.30 mL/min, with retention time of 3.50 and 3.62 min for internal standard tobramycin and analyte neomycin, respectively. The MS was performed with positive ionization mode, with data acquisition in Multi Reaction Monitor (MRM) mode. This method was proved to be specific, accurate (97.1-115% of nominal values) and precise (CV% 15%). Calibration curves for matrix matched standard of neomycin ranged from 1.25 to 200 μg/mL, with LOD and LLOQ of 0.625 and 1.25 μg/mL in blank matrix. The matrix effect was corrected to (-0.1) - 1.33 by adding internal standard. The relative SPE recovery values were ≥98.9% in low, medium and high QC samples. Neomycin in matrix proved to be stable under room temperature - and -20 °C, or under three freeze-thawing cycles, or under processing as well. Finally, the proposed method was successfully applied to a toxicokinetics study of neomycin in perilymph after round window membrane (RWM) administration, which was in accordance with threshold shift of auditory brainstem response (ABR) test related to hearing loss. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect