Inhibition of PLA2G4A Reduces the Expression of Lung Cancer-Related Cytokines
| Autor: | Xiumei Wang, Minghua Sui, Xuan Zhao, Weiwei Zhang, Liangming Zhang, Dengjun Sun, Jing Jiang, Chunxia Xin, Yanchun Wang, Ping Sui, Dongmei Geng |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
A549 cell Arachidonyl trifluoromethyl ketone biology medicine.medical_treatment Cell Prostacyclin Cell Biology General Medicine PLA2G4A Molecular biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Real-time polymerase chain reaction 030220 oncology & carcinogenesis Genetics medicine biology.protein lipids (amino acids peptides and proteins) Wound healing Molecular Biology medicine.drug Prostaglandin E |
| Zdroj: | DNA and cell biology. |
| ISSN: | 1557-7430 |
| Popis: | Phospholipase A2-IVA (PLA2G4A) is the most abundant subtype of cytoplasmic phospholipase A2 (cPLA2) and is an important enzyme in tumor development. Our study aimed to explore the role of PLA2G4A in the regulation of lung cancer. The contents of cell-related cytokines (microsomal prostaglandin E synthase-1 [mPGES], PGE2, and prostacyclin [PGI2]) in A549 cells were analyzed by ELISA kits. Cell counting kit-8 (CCK8) was used to detect the effects of inhibitor of cPLA2 (arachidonyl trifluoromethyl ketone [AACOCF3]) on the proliferation of A549 cells. The migration and invasion of A549 cells were tested by cell scratch wound healing assay and transwell assay, respectively. Real-time quantitative PCR and Western blotting were used to detect the effect of inhibitor AACOCF3 on the expression of related mRNA and protein in A549 cells. ELISA result showed that the levels of mPGES, PGE2, and PGI2 in control group were significantly higher than those in the AACOCF3 group. Cell inhibition rate in the control group was significantly lower than that in the AACOCF3 group. The percentage of wound healing in the control group was significantly higher than that in the AACOCF3 group. Meanwhile, the relative invasive number of cells in the control group was significantly higher than those in the AACOCF3 group. The expression levels of related mRNA of PLA2G4A and cyclooxygenase-2 (COX-2) and the expression levels of mPGES, COX-1, and COX-2 protein in the control group were significantly higher than those in the AACOCF3 group. Our research showed that PLA2G4A was involved in migration and invasion of lung cancer cells. |
| Databáze: | OpenAIRE |
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