Epigenetic engineering: histone H3K9 acetylation is compatible with kinetochore structure and function
| Autor: | Bergmann, J. H., Jakubsche, J. N., Martins, N. M., Kagansky, A., Nakano, M., Kimura, Hiroshi, Kelly, D. A., Turner, B. M., Masumoto, H., Larionov, V., Earnshaw, W. C. |
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| Rok vydání: | 2012 |
| Předmět: |
Human artificial chromosome
Chromosomal Proteins Non-Histone Recombinant Fusion Proteins Kinetochore assembly Centromere Chromosomal Proteins Non-Histone/metabolism Autoantigens Chromatin remodeling Chromosomes Artificial Human Autoantigens/metabolism Cell Line Epigenesis Genetic Histones Histone H3 Histone code Humans Kinetochores ChIA-PET Research Articles Transcription Factor RelA/genetics biology Lysine Transcription Factor RelA Acetylation Herpes Simplex Virus Protein Vmw65 Cell Biology Chromatin/chemistry/metabolism Molecular biology Chromatin Cell biology Kinetochores/chemistry/*physiology Kinetochore Histone biology.protein Epigenetics Histones/chemistry/*metabolism Herpes Simplex Virus Protein Vmw65/genetics CENP-A Centromere Protein A Centromere/*metabolism Lysine/metabolism |
| Zdroj: | Bergmann, J H, Jakubsche, J N, Martins, N M, Kagansky, A, Nakano, M, Kimura, H, Kelly, D A, Turner, B M, Masumoto, H, Larionov, V & Earnshaw, W C 2012, ' Epigenetic engineering: histone H3K9 acetylation is compatible with kinetochore structure and function ', Journal of Cell Science, vol. 125, no. 2, pp. 411-421 . https://doi.org/10.1242/jcs.090639 |
| ISSN: | 1477-9137 |
| DOI: | 10.1242/jcs.090639 |
| Popis: | Human kinetochores are transcriptionally active, producing very low levels of transcripts of the underlying alpha-satellite DNA. However, it is not known whether kinetochores can tolerate acetylated chromatin and the levels of transcription that are characteristic of housekeeping genes, or whether kinetochore-associated ‘centrochromatin’, despite being transcribed at a low level, is essentially a form of repressive chromatin. Here, we have engineered two types of acetylated chromatin within the centromere of a synthetic human artificial chromosome. Tethering a minimal NF-κB p65 activation domain within kinetochore-associated chromatin produced chromatin with high levels of histone H3 acetylated on lysine 9 (H3K9ac) and an ~10-fold elevation in transcript levels, but had no substantial effect on kinetochore assembly or function. By contrast, tethering the herpes virus VP16 activation domain produced similar modifications in the chromatin but resulted in an ~150-fold elevation in transcripts, approaching the level of transcription of an endogenous housekeeping gene. This rapidly inactivated kinetochores, causing a loss of assembled CENP-A and blocking further CENP-A assembly. Our data reveal that functional centromeres in vivo show a remarkable plasticity – kinetochores tolerate profound changes to their chromatin environment, but appear to be critically sensitive to the level of centromeric transcription. |
| Databáze: | OpenAIRE |
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