Frequency-dependent electrical detection of protein binding events
| Autor: | Tami L. Lasseter, Wei Cai, Robert J. Hamers |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Materials science
Surface Properties Analytical chemistry Biotin Biosensing Techniques Substrate (electronics) Glassy carbon Biochemistry Analytical Chemistry law.invention law Electric Impedance Electrochemistry Environmental Chemistry Electrical measurements Electrical impedance Spectroscopy biology Spectrum Analysis Avidin Dielectric spectroscopy Electrical network biology.protein Biosensor Protein Binding |
| Zdroj: | The Analyst. 129:3 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/b307591e |
| Popis: | Frequency-dependent electrochemical impedance spectroscopy has been used to characterize the changes in electrical response that accompany specific binding of a protein to its substrate, using the biotin-avidin system as a model. Our results show that avidin, at concentrations in the nanomolar range, can be detected electrically in a completely label-free manner under conditions of zero average current flow and without the use of any auxiliary redox agents. Impedance measurements performed on biotin-modified surfaces of gold, glassy carbon, and silicon were obtained over a wide frequency range, from 5 mHz to 1 MHz. On each biotin-modified surface, binding of avidin is most easily detected at low frequencies |
| Databáze: | OpenAIRE |
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