Gonadotropin-dependent regulation of bovine pituitary adenylate cyclase-activating polypeptide in ovarian follicles prior to ovulation

Autor:	Khampoune Sayasith, Kristy A. Brown, Jean Sirois
Rok vydání:	2007
Předmět:	Male Embryology Cyclic AMP-Dependent Protein Kinase Type II Chorionic Gonadotropin Human chorionic gonadotropin chemistry.chemical_compound Endocrinology Ovarian Follicle Testis Cloning Molecular Receptor Cells Cultured media_common Sulfonamides Forskolin Reverse Transcriptase Polymerase Chain Reaction Obstetrics and Gynecology Intestines Blotting Southern Follicular Phase Theca Pituitary Gland Theca Cells Pituitary Adenylate Cyclase-Activating Polypeptide Receptors Vasoactive Intestinal Peptide Type II Female Gonadotropin hormones hormone substitutes and hormone antagonists Ovulation endocrine system medicine.medical_specialty medicine.drug_class Granulosa cell media_common.quotation_subject Molecular Sequence Data Transfection Internal medicine medicine Animals Humans Amino Acid Sequence RNA Messenger Protein kinase A DNA Primers Granulosa Cells Base Sequence Colforsin Uterus Cell Biology Isoquinolines Cyclic AMP-Dependent Protein Kinases Molecular biology Rats Enzyme Activation Gene Expression Regulation Reproductive Medicine chemistry Cyclooxygenase 2 Cattle Sequence Alignment Gonadotropins Receptors Pituitary Adenylate Cyclase-Activating Polypeptide Type I
Zdroj:	Reproduction. 133:441-453
ISSN:	1741-7899 1470-1626
Popis:	To study the regulation of bovine pituitary adenylate cyclase-activating polypeptide (PACAP) in preovulatory follicles prior to ovulation, PACAP cDNA was isolated by RT-PCR. Its open reading frame (ORF) is composed of 531 bp, and encodes for a 176-amino acid protein that bears 76–90% identity with other PACAP homologs. Using bovine preovulatory follicles obtained between 0 and 24 h after human chorionic gonadotropin (hCG) and semiquantitative RT-PCR/Southern blot, we demonstrate that levels of PACAP mRNA were low at 0 h, markedly increased at 6 and 12 h (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a57044090371c9450b267cea254a412b https://doi.org/10.1530/rep-06-0188 Zobrazit plný text záznamu