Genomic characteristics and pathogenicity of natural recombinant porcine reproductive and respiratory syndrome virus 2 harboring genes of a Korean field strain and VR-2332-like strain
| Autor: | Jun Woo Park, Choi-Kyu Park, Taeyong Kwon, Sang Chul Kang, Young S. Lyoo, Sung J. Yoo |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Virus Cultivation
Swine animal diseases Porcine Reproductive and Respiratory Syndrome Genome Viral Virus Article law.invention 03 medical and health sciences law Virology Disease Transmission Infectious Pathogenicity Animals Porcine respiratory and reproductive syndrome virus Gene 030304 developmental biology Recombination Genetic 0303 health sciences Pig Korea biology Virulence Genetic heterogeneity Strain (biology) 030302 biochemistry & molecular biology respiratory system Viral Load Porcine reproductive and respiratory syndrome virus biology.organism_classification Recombination Cell culture Recombinant DNA Viral load |
| Zdroj: | Virology |
| ISSN: | 1096-0341 0042-6822 |
| Popis: | Porcine reproductive and respiratory syndrome (PRRS), an economically-important disease caused by PRRS virus (PRRSV), has become endemic to most pig-producing countries. Point mutation and recombination are responsible for genetic heterogeneity, resulting in circulation of genetically-diverse strains. However, no natural recombinant PRRSV has yet been identified in Korea. Here, we successfully isolated natural recombinant PRRSV-2 (KU-N1202) using cell culture, investigated its genomic characteristics, and further evaluated its pathogenicity. KU-N1202 is a recombinant strain between Korean MN184-like and VR-2332-like strains. Specifically, ORF5 to partial ORF7 of the VR-2332-like strain was inserted into the backbone of a CP07-626-2-like strain. KU-N1202 induced mild-to-moderate clinical signs and mild histopathological changes with low viral loads in challenged pigs. Contact pigs showed minimal clinical signs and lower viral loads than those in the challenge group. This study demonstrates the genomic characteristics and pathogenicity of natural recombinant PRRSV-2, illustrating the potential importance of recombination in the field. Highlights • A natural recombinant PRRSV-2 virus (KU-N1202) was isolated using cell culture. • The virus harbored the genes from field strain and VR-2332-like strain. • KU-N1202 induced mild-to-moderate clinical signs with low viral loads in challenged pig. • Contact pigs showed minimal clinical signs with relatively low viral loads. |
| Databáze: | OpenAIRE |
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