Induction of T → G and T → A transversions by 5-formyluracil in mammalian cells

G and T --> A transversions were the mutations found most frequently, suggesting the formation of 5-fU.C and 5-fU.T base pairs, respectively. This is the first report that clearly shows the induction of transversion mutations by an oxidized pyrimidine base in DNA in mammalian cells. -->

ISSN:	1383-5718
DOI:	10.1016/s1383-5718(01)00312-6
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a56f09b295ef96d595db5b4fe1af14be https://doi.org/10.1016/s1383-5718(01)00312-6
Rights:	CLOSED
Přírůstkové číslo:	edsair.doi.dedup.....a56f09b295ef96d595db5b4fe1af14be
Autor:	Naoko Murata-Kamiya, Naoko Karino, Yoshihito Ueno, Akira Matsuda, Hiroyuki Kamiya, Hiroshi Kasai
Rok vydání:	2002
Předmět:	Base pair Health Toxicology and Mutagenesis Molecular Conformation Nucleic acid sequence DNA replication Biology Molecular biology Thymine Restriction enzyme chemistry.chemical_compound chemistry Biochemistry Coding strand COS Cells Mutation Genetics Animals Uracil Transversion DNA Mutagens
Zdroj:	Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 513:213-222
ISSN:	1383-5718
DOI:	10.1016/s1383-5718(01)00312-6
Popis:	Oxidatively damaged thymine, 5-formyluracil (5-fU), was incorporated into a predetermined site of double-stranded shuttle vectors. The nucleotide sequences in which the modified base was incorporated were 5'-CFTAAG-3' and 5'-CTFAAG-3' (F represents 5-fU), the recognition site for the restriction enzyme AflII (5'-CTTAAG-3'). The 5-fU was incorporated into a template strand of either the leading or lagging strand of DNA replication. The modified DNAs were transfected into simian COS-7 cells, and the DNAs replicated in the cells were recovered and were analyzed after the second transfection into Escherichia coli. The 5-fU did not block DNA replication in mammalian cells. The 5-fU residues were weakly mutagenic, and their mutation frequencies in double-stranded vectors were 0.01-0.04%. The T --> G and T --> A transversions were the mutations found most frequently, suggesting the formation of 5-fU.C and 5-fU.T base pairs, respectively. This is the first report that clearly shows the induction of transversion mutations by an oxidized pyrimidine base in DNA in mammalian cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a56f09b295ef96d595db5b4fe1af14be https://doi.org/10.1016/s1383-5718(01)00312-6 Zobrazit plný text záznamu Full Text from ScienceDirect