Transcriptional Regulation of Rat Insulin-Like Growth Factor-Binding Protein-1 Expression by Growth Hormone
| Autor: | Liam J. Murphy, Jiangming Luo, Charita Seneviratne |
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| Rok vydání: | 1990 |
| Předmět: |
Male
medicine.medical_specialty Transcription Genetic medicine.medical_treatment Insulin-like growth factor-binding protein Endocrinology Internal medicine Gene expression Transcriptional regulation medicine Animals RNA Messenger Insulin-Like Growth Factor I Molecular Biology Hypophysectomy Messenger RNA biology Binding protein Insulin Rats Inbred Strains General Medicine Somatomedin Rats Insulin-Like Growth Factor Binding Proteins Kinetics Gene Expression Regulation Liver Growth Hormone Insulin-like growth factor 2 biology.protein Carrier Proteins DNA Probes |
| Zdroj: | Molecular Endocrinology. 4:1199-1204 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | The low mol wt insulin-like growth factor-binding protein (IGFBP-1) originally isolated from amniotic fluid has been considered to be GH independent. In this report we have examined the effect of GH on hepatic IGFBP-1 expression in the hypophysectomized rat. Using a rat IGFBP-1 cDNA probe we now demonstrated that hepatic IGFBP-1 expression is up-regulated in the GH-deficient rat. In addition, using ligand blotting, an increase in the abundance of a 30-kDa [125I]IGF-I-BP was detected in both hepatic extracts and serum from hypophysectomized rats. After a single ip injection of GH, the IGFBP-1 transcription rate was reduced within 30 min to the levels seen in the sham-operated control rats. Similarly, hepatic IGFBP-1 mRNA abundance was reduced after both acute GH injection and chronic GH treatment for 8 days. These data demonstrate that IGFBP-1 expression is inversely regulated by GH. |
| Databáze: | OpenAIRE |
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