Ca2+‐binding protein centrin 4 is a novel binding partner of rat Eag1 K+ channels
| Autor: | Chung Jiuan Jeng, Ting Feng Lin, Po Hao Hsu, Yi Chih Chiu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Retina Immunoprecipitation Biology Subcellular localization immunoprecipitation General Biochemistry Genetics and Molecular Biology Yeast neuron Cell biology Synapse 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure voltage‐gated K+ channel Centrin medicine Neuron EF‐hand calcium‐binding protein yeast two‐hybrid screening Research Articles K channels Research Article |
| Zdroj: | FEBS Open Bio |
| ISSN: | 2211-5463 |
| Popis: | Eag1 is neuron-specific K(+) channel abundantly expressed in the brain and retina. Subcellular localization and physiological analyses in neurons reveal that Eag1 may participate in Ca(2+)-signaling processes in the synapse. Here, we searched for rat Eag1 (rEag1)-binding proteins that may contribute to Ca(2+) regulation of the K(+) channel. Yeast two-hybrid screening identified centrin 4, a member of the centrin family of Ca(2+)-binding proteins. GST pull-down and immunoprecipitation assays in brain and retina lysates confirm the interaction of centrin with rEag1 in neurons. Centrin 4 binds to rEag1 in the absence of Ca(2+). Raising Ca(2+) concentration enhances the association efficiency of centrin 4 and rEag1, and is required for the suppression of rEag1 currents by centrin 4. Altogether, our data suggest that centrin 4 is a novel binding partner that may contribute to Ca(2+) regulation of rEag1 in neurons. |
| Databáze: | OpenAIRE |
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