Human umbilical vein and dermal microvascular endothelial cells show heterogeneity in response to PKC activation
| Autor: | Helen Yarwood, Justin C. Mason, D. O. Haskard, Katharine Sugars |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Umbilical Veins
Transcription Genetic Endothelium Physiology Vascular Cell Adhesion Molecule-1 Inflammation Biology Umbilical cord Umbilical vein Phorbol Esters medicine Humans RNA Messenger Cycloheximide Cells Cultured Phorbol 12 13-Dibutyrate Protein Kinase C Protein kinase C Skin Tumor Necrosis Factor-alpha Cell adhesion molecule Microcirculation Cell Biology Cell biology Enzyme Activation Endothelial stem cell Kinetics medicine.anatomical_structure Cell culture Thy-1 Antigens Endothelium Vascular medicine.symptom E-Selectin Cell Adhesion Molecules Cell Division |
| Zdroj: | American Journal of Physiology-Cell Physiology. 273:C1233-C1240 |
| ISSN: | 1522-1563 0363-6143 |
| Popis: | Changes in endothelial cell (EC) phenotype are central to the function of endothelium in inflammation. Although these events mainly occur in the microvasculature, previous studies have predominantly used large-vessel EC. Using enzyme-linked immunosorbent and flow cytometric assays, we compared the responses of human umbilical vein endothelial cells (HUVEC) and dermal microvascular endothelial cells (DMEC) to the activation of protein kinase C (PKC). Stimulation with phorbol 12,13-dibutyrate and more selective PKC agonists, including 12-deoxyphorbol 13-phenylacetate 20-acetate (dPPA), induced morphological changes and proliferation in both EC types. PKC activation induced a marked increase in Thy-1 expression on DMEC and only a moderate rise on HUVEC. Furthermore, heterogeneity in the induction of the adhesion molecules intercellular adhesion molecule 1, vascular cell adhesion molecule 1 (VCAM-1), and E-selectin between the two EC types following activation of PKC was demonstrated. In particular, E-selectin and VCAM-1 were significantly upregulated on HUVEC but not DMEC. The data indicate that the PKC pathway is unlikely to be important for E-selectin and VCAM-1 expression in the microvasculature but are consistent with a role for PKC in angiogenesis. This diversity in signaling in response to PKC activation may depend on differential utilization of PKC isozymes and may facilitate specialized endothelial responses. |
| Databáze: | OpenAIRE |
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